DNA甲基化介导的microRNA-33b下调及其在胃癌中的作用  

miR-33b down-regulation by DNA Methylation and its impacts on gastric cancer

作　　者：阴海鑫[1] 王斌[1] 王芳[2] 余佳[2] 赵浩亮[3] 

机构地区：[1]山西医科大学普外科,山西太原030032 [2]中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室,北京100005 [3]山西医科大学附属山西医学科学院山西大医院普外科,山西太原030032

出　　处：《基础医学与临床》2013年第10期1269-1274,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(91129716)

摘　　要：目的探讨miR-33b基因上游CpG岛甲基化对该基因在胃癌组织中表达情况的影响,分析其表达与临床统计学资料间的关系。方法使用Taqman real-time PCR法检测miR-33b在42例胃癌患者癌及癌旁组织中的相对表达情况,并分析miR-33b表达水平与胃癌临床病理特征间的关系,随后使用组织基因组DNA快速提取试剂盒提取胃癌组织及正常人血液基因组DNA,经重亚硫酸氢盐处理后,甲基化特异性PCR法检测其上游CpG岛的甲基化程度,PCR产物经琼脂糖凝胶检测,并进行统计分析。结果 miR-33b表达下调与胃癌患者的年龄、性别、发生部位、有无脉管转移、肿瘤分型无相关性,但与肿瘤是否发生远处转移具有相关性(P<0.05),且其低表达与上游CpG岛的甲基化沉默修饰密切相关(P<0.05)。结论 miR-33b的表达在胃癌中受甲基化调控且与胃癌远处转移相关。Objective To investigate the impacts of miR-33b down-regulation by DNA Methylation in gastric cancer （GC） and the association between miR-33b level and various clinieopathological factors. Methods Expression of miR-33b was detected by Taqman real-time PCR in 42 paired GC samples. The association between miR-33b level and various clinicopathological factors was analyzed. Genomic DNA samples were amplificated by PCR after modifica- tion by sodium bisulfite using the EpiTect Bisulfite Kit then the methylation degree of CpG island upstream of miR- 33b gene was detected by methylation specific PCR （MSP）. Results The lower level expression of miR-33b was not associated with gender, age, venous invasion, position, borrmann typing, pT stage, pN stage, but significantly asso- ciated with gastric cancer metastasis （ P 〈 0. 05） and regulated by CpG island hypermethylation （ P 〈 0. 05）. Conclu- sions miR-33b may be regulated by DNA methylation and act as a tumor suppressor in gastric cancer.

关 键 词：miR-33b 胃癌 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]