可见光漫反射光谱监测大鼠脑缺血及其意义  

Monitoring of rat brain ischemia degree with visible light diffuse reflectance spectra and its significance

作　　者：傅楚华[1] 王海峰 李钊[1] 李飞[1] 邹永杰[1] 黄宇星[1] 张大勇 冯华[1] 

机构地区：[1]第三军医大学附属西南医院神经外科民、中国工程物理学院与第三军医大学附属西南医院物理生物交叉实验室,重庆400038 [2]中国工程物理学院流体物理研究所

出　　处：《中华创伤杂志》2013年第9期877-881,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金面上资助项目（30973100）;重庆市自然科学基金重点资助项目（CSTC2012jjB0081）

摘　　要：目的 观察可见光漫反射光谱监测大鼠正常、缺血及坏死脑组织的缺血严重程度,为术中实时监测脑灌注提供研究基础. 方法 选择36只SD大鼠并按随机数字表法分为正常组及缺血1,3,6,12,24h组,每组6只,缺血组采用线栓法制备大脑中动脉梗死模型,光谱仪测量各组标本可见光漫反射光谱,计算其平均漫反射光谱比值（R630/R545）. 结果 缺血时间越长,梗死脑组织的光谱反射率越高,542 nm及577 nm波谷越浅,且缺血12,24 h脑组织在630 nm处出现较小波谷.除缺血12h与24 h组间外,其余各组间R630/R545差异均有统计学意义（P＜0.05）.结论 可见光漫反射光谱可以实时监测并鉴别正常、缺血及坏死脑组织甚至脑组织的缺血严重程度,为临床术中实时监测脑灌注提供基础.Objective To observe role of the visible light diffuse reflectance spectra in monitoring the severity of brain ischemia in normal,ischemic and necrotic rat brain tissues so as to offer research basis for intraoperative real-time monitoring of brain reperfusion.Methods Thirty-six SD rats were randomly divided into noumal group and ischemia 1 hour,3 hours,6 hours,12 hours and 24 hours groups,with 6 rats per group.Models of middle cerebral artery occlusion were established using thread technique in ischemic groups.Visible light diffuse reflectance spectroscopy was detected by a spectrometer and mean diffuse reflectance spectroscopy ratio （R630/R545） was measured.Results Spectral reflectance of infarcted brain tissue was enhanced with the prolonged ischemic time while the trough of the spectra at 542 nm and 577 nm was shallowed.Moreover,small trough was appeared at 630 nm at 12 h and 24 h after ischemia.Diffuse reflectance spectroscopy ratio （R630/R545） showed statistical difference among all groups （P 〈 0.05） with the exception between ischemic 12 hours and 24 hours groups.Conclusion Visible diffuse reflectance spectroscopy can timely monitor and discriminate the normal,ischemic and necrotic brain tissues and even the severity of brain ischemia and hence lay a research basis for intraoperative real-time monitoring of brain reperfusion.

关 键 词：梗死 大脑中动脉 脑缺血 大鼠 漫反射光谱 

分 类 号：R743[医药卫生—神经病学与精神病学]