实验性牙周炎大鼠胸主动脉平滑肌细胞中ICAM-1的表达  

Expression of Intercellular Adhesion Molecule-1 in Thoracic Aorta Smooth Muscle Cells of Rats with Periodontitis

作　　者：林江[1] 代佳音[1] 王丽[2] 毕佳锐[3] 刘玉三[1] 王家宏[2] 毕良佳[1] 

机构地区：[1]哈尔滨医科大学附属第四医院口腔科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院 [3]武汉大学附属口腔医院

出　　处：《口腔医学研究》2013年第9期796-798,802,共4页Journal of Oral Science Research

基　　金：黑龙江省教育厅基金资助项目(编号:11511230)

摘　　要：目的:观察细胞间粘附分子-1(ICAM-1)在实验性牙周炎大鼠胸主动脉平滑肌细胞中的表达。方法:SD雄性大鼠随机分为实验组和对照组,每组10只。实验性牙周炎组用粘性饲料喂养,丝线结扎上颌第二磨牙,局部涂布P.gingivalis菌液,对照组用普通饲料喂养,4周后处死,取大鼠上颌牙齿、牙周组织及胸主动脉,免疫组织化学方法检测实验组织中ICAM-1的表达情况。结果:对照组血管平滑肌细胞(VSMC)中未见ICAM-1的表达,实验组VSMC中ICAM-1呈阳性表达。结论:实验性牙周炎大鼠胸主动脉平滑肌细胞中可见ICAM-1表达,这提示牙周炎的发生、发展过程可能影响血管壁的炎症反应,从而在动脉粥样硬化的发生、发展中有重要的意义。Objective： To obsere the expression of adhesion molecule--1 （ICAM--1） in thoracic aorta smooth mus- cle cells（VSMC） of rats with periodontitis. Methods： SD male rats were randomly divided into experimental group and control group, with ten rats each group. The experimental group rats were fed with viscous diet, iigatured with silk and added with P. gingivalis. The control ones were fed with normal diet. Four weeks later, the rats were sacri- fie＆ The tissue around maxillary second molar and the thoracic aorta tissue of rats were collected and processed. The expression of ICAM-1 in smooth muscle cells were detected by immunohistochemistry. Results： There was no expression of ICAM-1 in VSMC of control group but high expression in experimental group. Conclusion： There is expression of ICAM-1 in VSMC of rats, and periodontitis pathogenesis might be involved in inflammation of blood vessel walls, which may play an important role in the progression of atherosclerosis.

关 键 词：牙周炎 动脉粥样硬化 平滑肌细胞 细胞间粘附分子-1 

分 类 号：R781.4[医药卫生—口腔医学]