琼枝野生群体与养殖群体的EST-SSR分析  

EST-SSR analysis of wild and farmed Betaphycus gelatinum

作　　者：胡吟胜[1] 段泽林[2] 黄勃[1] 于淑楠[1] 王婷[3] 

机构地区：[1]海南大学海洋学院,海南海口570228 [2]华东师范大学生命科学学院,上海200241 [3]中国科学院海洋研究所,山东青岛266071

出　　处：《水产学报》2013年第9期1313-1318,共6页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA10A412-8);海南大学植物学国家重点学科(071001);海南省重点科技项目(080137);国家自然科技基金项目(41176084);国家科技支撑计划项目(2009BADB2B0404-02);海洋公益行业科研专项(201105008-7);中医药行业科研专项(201207002-03)

摘　　要：为研究野生琼枝与养殖琼枝的遗传差异,利用EST-SSR技术分析了海南东北部地区9株野生和9株养殖琼枝的遗传差异性。结果显示,用5对EST-SSR引物对18个个体的基因组进行扩增,扩增出的片段大小为250～2 500 bp,琼枝野生群体和养殖群体的多态性比例分别为74.29%和70.00%;野生群体之间的平均遗传距离为0.46,养殖琼枝群体之间的平均遗传距离为0.22。聚类分析图显示,在相似系数0.49处,18株群体按野生与养殖分为2大类,在相似系数0.66附近,野生群体分成3类,养殖琼枝群体分为2类,在相似系数0.73附近,野生琼枝群体分成5类,而养殖群体为3类,相似系数越高,野生群体比养殖群体分类单元越多。研究表明,野生琼枝群体的遗传多样性比养殖琼枝群体高。EST-SSR was used to detect the hereditary difference in wild and cultured populations of the Betaphycus gelatinae,the result showed that nine individuals from each population were used of the 5 EST-SSR primers amplified,a total of 195 reproducible loci ranging from 250 to 2 500 bp were amplified from all the 18 individuals.The average percentages of polymorphic loci in wild and cultured populations were 74.29% and 70%.The intra-population genetic distance was 0.46 in wild population and 0.22 in cultured population.The cluster analysis showed that 18 individuals were clustered into two groups base on 0.49 of GS;wild populations were divided into 3 branches and cultured populations were divided into 2 branches base on 0.66;wild populations were divided into 5 branches and cultured populations were divided into 3 branches base on 0.73.The higher the value,the more branches the wild populations have.The research shows that genetic diversity of wild populations ampler than cultured

关 键 词：琼枝 EST-SSR 野生 养殖 

分 类 号：S965.322[农业科学—水产养殖]