^(188)Re标记叶酸偶联白蛋白纳米微球对SKOV_3人卵巢癌生长抑制作用研究  

The Inhibitory Effects of ^(188)Re-Labeled Folate Coupling with Magnetic Albumin Nanoparticles on SKOV_3 Ovarian Cancer in Vivo

作　　者：唐秋莎[1] 陈道桢[2] 臧嘉[2] 郭彩琴[2] 

机构地区：[1]东南大学医学院病理与生理学系,南京210009 [2]南京医科大学附属无锡妇幼保健院中心实验室

出　　处：《天津医药》2013年第10期978-980,I0003,共4页Tianjin Medical Journal

基　　金：国家自然科学基金项目资助(项目编号:30872999);江苏省"科教兴卫工程"专项经费资助项目(项目编号:RC2011033)

摘　　要：目的观察核素标记叶酸靶向白蛋白纳米微球(188Re-folate-CDDP/HAS MNP)对SKOV3人卵巢癌细胞生长作用的影响。方法建立人卵巢癌细胞SKOV3裸鼠模型,并将64只荷瘤鼠随机分成8组,每组8只,分别为(A)阴性对照组;(B)采用CDDP方案化疗的单纯化疗组;(C)核素靶向内照射的单纯放疗组;(D)磁感应热疗的单纯热疗组;(E)化疗联合放疗组;(F)化疗联合热疗治疗组;(G)放疗联合热疗治疗组;(H)热疗、化疗、放疗联合治疗组。各组经治疗后,观察肿瘤生长增殖情况,计算肿瘤质量抑制率,观察肿瘤组织病理变化。结果各治疗组的肿瘤质量均低于对照组(P<0.05),且与其他治疗组相比,热疗、化疗、放疗联合治疗组的肿瘤质量最低(P<0.05)。结论磁感应热疗、化疗、核素靶向内照射放疗的联合作用能有效抑制卵巢癌的生长,对卵巢癌治疗具有潜在应用前景。Objective To investigate the effects of isotope labeled folate targeting albumin nanoparticles （188Re-fo- late-CDDP/HAS MNP） on human SKOV3 ovarian cancer cells in vivo. Methods The human SKOV3 ovarian cancer model was established in mice. Sixty-four tumor-bearing mice were randomly divided into eight groups： （A） negative control group, （B） chemotherapy group, （C） radiotherapy alone group, （D） hyperthermia alone group, （E） chemotherapy combined with radio- therapy group, （F） chemotherapy combined with hyperthermia therapy group, （G） radiotherapy combined with hyperthermia therapy group and （H） hyperthermia, chemotherapy and radiotherapy combined treatment group. After treatment, the cell pro- [iferation and tumor growth were observed. The inhibitory rate of tumor mass was measured. The histopathological changes of tumor were observed in all groups. Results The quality of tumor was significantly lower in treatment groups than that of control group （P 〈 0.05）. There was the lowest quality of tumor in hyperthermia, chemotherapy and radiotherapy combined treatment group than that of other treatment groups （P 〈 0.05）. Conclusion The combination of magnetic induction hyper- thermia, chemotherapy, targeted radionuclide of radiation exposure can effectively inhibit the growth of ovarian cancer, which has the potential application for ovarian cancer treatment.

关 键 词：顺铂 叶酸 纳米粒子 白蛋白类 卵巢肿瘤 癌 放射疗法 透热疗法 抗肿瘤联合化疗方案 

分 类 号：R737.31[医药卫生—肿瘤]