非小细胞肺癌FUS2基因SNPs多态性检测及其相关性的研究  

Polymorphism of FUS2 SNPs in non-small cell lung cancer and its clinical significance

作　　者：周碧燕[1] 李友邕[1] 赵丽萍[1] 魏威[2] 

机构地区：[1]南宁市第一人民医院检验科,广西南宁530022 [2]南方医科大学生物技术学院,广东广州510515

出　　处：《中华肿瘤防治杂志》2013年第18期1410-1412,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：南宁市科学研究与技术开发计划(20103132)

摘　　要：检测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者FUS2SNP s(767A/T)的多态性,并探讨其相关的临床意义。方法:收集2010-01-2011-12治疗的NSCLC患者84例,另选取体检科的健康体检者20名作为正常对照。SNPs多态性测定使用Real-Time PCR Taqman分析。结果:NSCLC患者A/A基因型频率为30.9%,A/T为35.7%,T/T为33.3%,对照组中分别为20.0%、55.0%和25.0%,A/A和T/T基因型在两组之间差异无统计学意义,P>0.05;A/T基因型在NSCLC组为55.0%,对照组为35.7%,两组之间具有统计学差异,χ2=4.62,P<0.05;NSCLC患者男和女性A/T基因型频率为37.0%和34.2%,差异无统计学意义,χ2=0.64,P>0.05;吸烟和不吸烟A/T基因型频率为38.9%和33.3%,差异无统计学意义,χ2=0.89,P>0.05;而在组织学分化程度上,高分化和中低分化A/T基因型频率为47.1%和28.0%,差异有统计学意义,χ2=3.89,P<0.05;淋巴结转移无和有A/T基因型频率为50.0%和25.0%,差异有统计学意义,χ2=4.35,P<0.05;临床病理分期Ⅰ、Ⅱ期和Ⅲ、Ⅳ期A/T基因型频率为45.5%和25.0%,差异有统计学意义,χ2=3.66,P<0.05。结论:FUS2SNP s(767A/T)基因型与NSCLC的易感性密切相关,可在NSCLC的早期诊断诊断、临床分级以及预后评估等方面起到一定的作用。To investigate the polymorphism of FUS2 SNP s（767A/T） in non-small cell lung cancer（NSCLC） and explore its clinical significance. METHODS： Totally 84 patients with NSCLC were enrolled in our study and another 20 healthy person were as a normal control. The genotypes of FUS2 SNP s（767A/T） was performed using the standard Taq- Man assays. RESULTS： The A/A, A/T, and T/T genotypes of FUS2 SNP s 767 in NSCLC group were accounted for 30.9%,35.7% and 33.3%,respectively; and which in control group were accounted for 20.0% ,55.0% and 25.0% ,re- spectively. No significant difference of A/A and T/T genotypes was found between NSCLC and control group（P〉0.05）. However,there was significantly difference of A/T genotype between the two groups（35.7 % vs 55.0 %;χ2 = 4.62, P〈 0.05）. The C/T genotype was not significant related with gender and smoking （P〉0.05） but it was significant related with histological grade（47.1% vs 28.0% ; χ2 =3.89 ,P〈0.05） ,lymphatic metastasis（50.0% vs 25.0% ; χ2=4.35 ,P〈 0.05） ,and clinical pathologic stage（45.5% vs 25.0%; χ2 = 3.66, P〈0.05） in NSCLC patients（P〈0.05）. CONCLU- SIONS： FUS2 SNP s（767A/T） genotype may be a susceptibility genotype in NSCLC. The detection of A/T genotype may be used as a mark for diagnosis in estimating the degree of differentiation or prognosis in NSCLC patients.

关 键 词：癌 非小细胞肺 FUS2基因 单核苷酸多态性 相关性分析 

分 类 号：R734.2[医药卫生—肿瘤]