骨髓单核细胞移植对脑梗死小鼠白质损伤及血管再生的影响  

The effect of transplanted bone marrow mononuclear cells for white matter damage and angiogenesis in cerebral infarction mice

作　　者：王建平[1] 王新新[1] 蒋超[1] 王梦涵[1] 余列[1] 陈明[1] 欧春影[1] 付晓杰[1] 韩伟[1] 周晨光[1] 

机构地区：[1]郑州大学第五附属医院神经内科郑州大学脑血管病研究所,郑州450052

出　　处：《中华行为医学与脑科学杂志》2013年第8期680-683,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81271284）

摘　　要：目的 探讨评价骨髓单个核细胞（bone marrow mononuclear cells,BMMNCs）移植对大脑中动脉栓塞小鼠脑白质损伤的疗效及梗死周围血管再生的影响.方法 线栓法将成年雄性小鼠制成大脑中动脉栓塞模型,模型成功后按随机表法随机分成假手术组,MCAO组,PBS组及BMMNCs移植组（BMMNCs组）.梯度密度离心法提取分离BMMNCs,模型成功后24 h经尾静脉移植入0.5 ml含1×106个BMMNCs细胞悬液,PBS组给予等体积的PBS溶液,神经功能学评分（mNSS）评价小鼠神经功能,luxol fast blue（LFB）染色观察梗死周围脑白质损伤情况,免疫荧光染色观察梗死周围血管再生情况.结果 除假手术组外,其他各组均有不同程度的神经功能缺损.梗死后56 d,BMMNCs组LFB着色减少百分比[（24.06±5.48）％]明显低于MCAO组[（62.22±5.38）％]及PBS组[（61.22±5.48）％],BMMNCs组血管密度[（249.8±11.8）个/mm2]明显高于MCAO组[（119.1 ±11.2）个/mm2]及PBS组[（114.3±12.1）个/mm2],差异有统计学意义（P＜0.05）,MCAO组与PBS组相比,LFB着色减少百分比和血管密度差异无统计学意义（P＞0.05）.结论 BMMNCs移植可促进血管新生,增加梗死周围区血管密度,减轻脑白质损伤程度,显著改善脑梗死小鼠神经功能.Objective To evaluate the effect of transplanting bone marrow mononuclear cells （BMMNCs）for white matter damage and angiogenesis in cerebral infarction mice.Methods The male adult mice were randomly divided into four groups：Sham operated group,MCAO group,PBS group and BMMNCs transplantation group （BMMNCs group）.The MCAO mice in last three groups were induced by occlusion of the right middle cerebral artery using an intra luminal filament technique.The successful model was injected with 0.5 ml cell suspension containing 1 × 106 BMMNCs via tail vein after 24h,and the PBS group were injected with the same volume of PBS.The effect of bone marrow mononuclear cells transplantation in mice were observed by recording the neurological scores,using the luxol fast blue （LFB） staining to assess the severity of white matter damage in peri-infarction zone,and the vWF immunofluorescence to investigate the vessel density in peri-infarction zone.Results Except for the sham operated group,the other groups suffered from different level of neurological deficits.After the 56 days of stroke,the reduced percent of white matter marked by LFB in BMMNCs group （（24.06 ± 5.48） %） was significantly lower（P 〈 0.05） than that in MCAO group （（62.22 ± 5.38） %） and that in PBS group （（61.22 ±5.48） %）,and the density of vessel in peri-infarction zone in BMMNCs group （249.8 ± 11.8/mm2） was significantly lower（P 〈 0.05） than that in MCAO group （119.1 ± 11.2/mm2） and that in PBS group （114.3 ± 12.1/mm2）.No difference was showed between the MCAO group and PBS group（P〉 0.05）.Conclusion Intravenously transplanted BMMNCs can promote the angiogenesis,increase the density of vessel in peri-infarction area,alleviate the white matter damage and stimulate the recovery of neurological function.

关 键 词：脑梗死 骨髓单个核细胞 移植 脑白质损伤 缺血性脱髓鞘 

分 类 号：R743.3[医药卫生—神经病学与精神病学]