健脾止动汤对多发性抽动症模型小鼠抽动行为及纹状体内氨基酸递质的影响  

Influence of Jianpizhidong decoction on the behavioral record an bilateral striatal amino acid neurotrans-mitters in mice with Tourette syndrome disease

作　　者：张雯[1,2] 王素梅[1] 王道涵[1] 卫利[1] 李珉景[1] 

机构地区：[1]北京中医药大学东方医院儿科,北京100078 [2]泰山医学院

出　　处：《中华行为医学与脑科学杂志》2013年第8期700-703,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：北京中医药大学自主选题项目（2011JYB22XS-030）

摘　　要：目的 研究健脾止动汤对多发性抽动症（Tourette syndrome,TS）模型小鼠抽动行为及纹状体内氨基酸递质的影响,探讨健脾止动汤的作用机制.方法 40只小鼠分成四组：空白对照组、模型组、健脾止动汤组（中药组）、泰必利组（西药组）.除空白对照组外,其余三组采用亚氨基二丙氰（IDPN）腹腔注射建立TS小鼠模型.造模成功后,模型小鼠进行行为学测试并重新分组.空白对照组和模型组给予生理盐水灌胃,西药组和中药组分别给予泰必利（50 mg/kg）和健脾止动汤汤（20 g/kg）,连续给药6周后,对小鼠再次进行行为学测试并用高效液相色谱法（HPLC）检测小鼠双侧纹状体内氨基酸递质的含量.结果 行为学结果显示,中药组、西药组治疗前（1.90±1.80,2.05±1.40）与治疗后（1.15±1.67,1.00±1.08）比较,差异有统计学意义（P＜0.05）.与空白对照组比较,模型组、西药组小鼠Glu（1858.33 ±203.00,1744.53±234.01）和GABA（261.24±47.31,258.57±57.64）均呈高表达状态（P＜0.05）,中药组Glu含量（1595.88±218.23）与模型组比较,差异有统计学意义（P＜0.05）,其余各组之间比较差异无统计学意义（P＞0.05）.模型组、西药组、中药组Glu/GABA（7.32±1.49,6.90±0.85,7.38±1.08）与空白对照组（7.55±0.75）相比,均有下降趋势,但各组之间比较差异无统计学意义（P＞0.05）.结论 健脾止动汤可能通过调节纹状体内Glu、GABA含量的变化,保持两者的动态平衡而发挥抗抽动效应.Objective To study the influences of Jianpizhidong decoction（JPZDD） on bilateral striatal amino acid neurotransmitters in mice with Tourette Syndrome （TS） disease and discuss the mechanism of JPZDD.Methods Thirty mice were injected 3,3-iminodipropionitrile （IDPN） with 350 mg· kg-1 once a day for 7 consecutive days except ten mice of the blank control group which were injected with saline.TS mice were randomly divided into three groups：TS model group,JPZDD group （the Chinese herbal group）,Tiapride group （the western medicine group）.The TS model group and the blank control group were gavaged with saline（0.9%）at 20 ml ·Kg-1,the Tiapride group with Tiapride at 50 mg · kg-1,and JPZDD group with JPZDD at 20 g · kg-1,respectively,once a day for six weeks.Then behavioral recordings were conducted,and the content of glumatic acid （Glu） and γ-aminobutyric acid （GABA） in the bilateral striatum of the mice were detected by using HPLC.Results Behavioral recording indicated that there were significant differences in the Chinese herbal group and the Tiapride group before（1.90 ± 1.80,2.05 ± 1.40） and after（1.15 ± 1.67,1.00 ± 1.08） treatment （P 〈 0.05）.The content of Glu and GABA in TS model group （1858.33 ± 203.00,261.24 ± 47.31） and Tiapride group （1744.53 ± 234.01,258.57 ± 57.64） were higher compared with the blank control group （P 〈 0.05）,and the content of Glu in JPZDD was significantly higher than that in TS model group.There was no statistical difference between other groups（P〉0.05）.The ratio of Glu and GABA were lower in the JPZDD,the Tiapride and the model groups than that in the blank control group,but there were no statistical differences between these groups （P〉 0.05）.Conclusion JPZDD can regulate the content of Glu and GABA in the striatum,and keep the dynamic equilibrium,which might be its pharmacodynamic mechanisms for treating TS.

关 键 词：健脾止动汤 多发性抽动症模型 谷氨酸 Γ-氨基丁酸 纹状体 小鼠 

分 类 号：R749.94[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]