丙戊酸钠孤独症模型鼠海马脑源性神经营养因子阳性神经元表达及锥体细胞形态学的变化  

The changes of brain-derived neurotrophic factor positive neurons and the morphology of pyramidal cells in hippocampal in sodium valproate induced autism rats

作　　者：衣明纪[1] 马小旭[2] 李音[3] 

机构地区：[1]青岛大学医学院附属医院儿童保健科,青岛266003 [2]东营市胜利油田中心医院 [3]青岛市妇女儿童医疗保健中心

出　　处：《中华行为医学与脑科学杂志》2013年第8期704-706,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：青岛大学医学院附属医院2010年院内重点培育学科专项建设基金项目（2010610）

摘　　要：目的 观察丙戊酸钠（VPA）孤独症模型鼠海马脑源性神经营养因子（BDNF）阳性神经元表达及锥体细胞形态学改变.方法 按Schneider方法制作VPA孤独症动物模型,采用免疫组化和图像分析技术检测模型鼠海马CA1区BDNF阳性神经元表达水平及海马CA1区锥体细胞形态学的改变.结果 孤独症模型组与正常对照组比较,海马CA1区锥体细胞BDNF阳性神经元表达水平增强,孤独症模型组与正常对照组阳性细胞数分别为（5.00±1.60）/视野和（3.00±1.04）/视野,差异有统计学意义（t=3.63,P=0.0015）；海马CA1区锥体细胞形态学显示,孤独症模型鼠海马CA1区锥体神经元发生凋亡增加.结论 孤独症的发病可能与海马CA1区锥体细胞BDNF表达水平以及锥体神经细胞的凋亡有关.Objective To explore the pathogenesis of autism by observation of changes of brain-derived neurotrophic factor（BDNF） positive neurons and the morphology of pyramidal cells in hippocampal CA1 region,and provide theoretical evidence for the therapeutic schedule.Methods Animal model of autism was obtained by Schneider method.Using the immunohistochemistry methods and image analysis,the number of BDNF positive neurons was examined in hippocampal CA1 region of the autism model rats and the normal rats,and the changes of pyramidal cell were observed in hippocampal CA1 region after HE staining.Results The numbers of BDNF positive neurons in the hippocampal CA1 region of the autism model rats were more than those of the normal rats （5.00 ±1.60 vs 3.00 ± 1.04,t =3.63,P =0.0015）.The morphology of pyramidal cells showed that the pyramidal cells of the autism model rats in hippocampal CA1 region had apoptosis.Conclusion The occurrence of autism may be related to the changes of BDNF and the morphology of pyramidal cells in hippocampal CA1 region.

关 键 词：孤独症谱系障碍 脑源性神经营养因子 锥体神经元 大鼠 

分 类 号：R749.94[医药卫生—神经病学与精神病学]