竹节参总皂苷提取物质量控制方法研究  

Quality Control Methods for Total Saponins Extract of Panax japonicus

作　　者：张海滨[1] 张长城[1] 关乔中[2] 何毓敏[1] 袁丁[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《中国实验方剂学杂志》2013年第18期68-70,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31070314)

摘　　要：目的:建立以活性主成分定量的竹节参总皂苷活性相关质量评价方法。方法:通过优化得到的竹节参总皂苷提取工艺制备竹节参总皂苷提取物,高效液相色谱法测定提取物中主成分含量,将这些主成分配制成与药材提取物总皂苷中含量相当浓度的主成分群,以体外抗氧化模型评价主成分群与提取物活性成分差异。结果:总皂苷提取物体外抗氧化活性为71.30 U·g-1,其中竹节参皂苷V含量24.9 mg·g-1、竹节参皂苷Ⅳa含量为90.6 mg·g-1、竹节参皂苷Ⅳ含量为38.7 mg·g-1、Pjs-2含量为41.1 mg·g-1,主成分群体外抗氧化能力为64.79 U·g-1。结论:以竹节参总皂苷主成分竹节参皂苷V、竹节参皂苷Ⅳa、竹节参皂苷Ⅳ、Pjs-2作为药效评价指标的准确性高,具有一定的科学性、合理性,竹节参总皂苷供试品中竹节参皂苷V含量应≥24.9 mg·g-1,竹节参皂苷Ⅳa含量≥90.6 mg·g-1,竹节参皂苷Ⅳ含量≥38.7 mg·g-1,Pjs-2含量≥39.6 mg·g-1。Objective： To establish total saponins from Panax japonicas（SPJ） activity related to quality standards by the quantification of main active ingredient.Method： The extraction process of total saponins from P.japonicas（SPJ） was optimized to obtain total saponin extraction.HPLC was used to determine the content of the main ingredients in the standard extract.The main ingredients were made into the preparations of principal component cluster equal with total saponins of herbal extracts to evaluate the differences between the principal component cluster and the active ingredients of herbal extraction through vitro antioxidant model.Result： Vitro antioxidant activity of standard extracts was 71.30 U·g-1;the content of saponin V of P.japonicus was 24.9 mg·g-1;saponin Ⅳa was 90.6 mg·g-1;saponin Ⅳ was 38.7 mg·g-1;Pjs-2 was 41.1 mg·g-1.Vitro antioxidant activity of the principal component cluster is 64.79 U·g-1.Conclusion： The method taking saponin V,saponin Ⅳa,saponin Ⅳ and Pjs-2 from P.japonicus as the principal component of the Panax japonicus total saponins has a high accuracy,which is scientific and reasonable.In the samples of Panax japonicus total saponins,the content of saponin V is no less than 24.9 mg·g-1,saponin Ⅳa is no less than 90.6 mg·g-1,saponin Ⅳ is no less than 38.7 mg·g-1,Pjs-2 is no less than 39.6 mg·g-1.

关 键 词：竹节参总皂苷 主成分群 体外抗氧化 质量控制 

分 类 号：R284.1[医药卫生—中药学]