FOLIN-酚比色法测定裸花紫珠中总酚含量  

Determination of Total Polyphenol from Callicarpa nudiflora by Folin-phonel Colorimetry

作　　者：颜小捷[1] 谷陟欣 卢凤来[1] 黄胜 宁德生[1] 李典鹏[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西桂林541006 [2]九芝堂股份有限公司,长沙410021 [3]海南九芝堂药业有限公司,海口570311

出　　处：《中国实验方剂学杂志》2013年第18期74-78,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(SQ2010BAJY1411-07-05);海南省中药现代化专项(2012ZY020);广西科学研究与技术开发计划项目(桂科攻11107010-3-10)

摘　　要：目的：建立裸花紫珠总酚的含量测定方法。方法：采用Folin-酚比色法测定裸花紫珠中总酚的含量,确定以没食子酸为对照品的最佳测定条件。结果：Folin-酚用量为0.5 mL,7.5%Na2CO3用量为1.5 mL,反应条件为40℃下加热10 min,显色条件为25℃下显色60 min,没食子酸浓度在0~18μg时与其吸光度有良好线性关系,其回归方程Y=32.262X-0.001 2（n=7）,r=0.999 9,根据此线性回归方程进行裸花紫珠总酚的含量测定。结论：该方法简单方便、稳定性好、准确度高,可用于裸花紫珠原料及其制剂的质量控制和定量分析。Objective： To establish a method for determination of total polyphenol from Callicarpa nudiflora.Method： The total polyphenol content of C.nudiflora was analyzed by folin-phonel colorimetry.The optimum conditions were investigated using gallic acid as standard.Result： The polyphenol content of C.nudiflora could be well calculated by applying folin-phonel reagent 0.5 mL,7.5% Na 2 CO 3 1.5 mL,reacted 10 min at 40 ℃,color condition 60 min at 25 ℃ for colorimetric reaction.The linear range of standard curve was 0-18 μg.The regression equation was found as follows Y = 32.262X-0.001 2（n = 7） with the correlation coefficent r = 0.999 9.Conclusion： The method established is simple,stable and accurate,which is suitable for analysis of total polyphenol and quality control in C.nudiflora.

关 键 词：裸花紫珠 总酚 福林-酚 

分 类 号：R284.1[医药卫生—中药学]