PTEN、Survivin基因蛋白在宫颈癌变过程中的联合表达及其相关性研究  

Expressions of PTEN and Survivin in the progression of cervical neoplasia and their clinical significances

作　　者：卢丹 尹香花 肖芹 王翠梅 

机构地区：[1]扬州大学临床医学院妇产科,江苏扬州225001 [2]扬州大学临床医学院病理科,江苏扬州225001

出　　处：《中国妇幼保健》2013年第28期4721-4724,共4页Maternal and Child Health Care of China

摘　　要：目的:探讨抑癌基因蛋白PTEN和凋亡抑制基因蛋白Survivin在宫颈癌变过程中的联合表达。结合宫颈病变的临床病理学特征,对两者基因蛋白阳性表达的相关性进行临床研究。方法:选取20例宫颈糜烂伴鳞状上皮化生、30例宫颈上皮内瘤变以及49例宫颈鳞状细胞癌患者,应用免疫组化SP法检测各组宫颈组织中抑癌基因蛋白PTEN和凋亡抑制基因蛋白Survivin的表达。选取10例正常宫颈组织作为对照组。结果:①在宫颈癌变动态过程中,从宫颈糜烂伴鳞状上皮化生、宫颈上皮内瘤变到宫颈鳞状细胞癌,抑癌基因PTEN的表达逐渐减弱(P<0.05)。而凋亡抑制基因蛋白Survivin表达则逐渐增强(P<0.05)。②PTEN在宫颈癌中的阳性表达与临床分期、病灶大小有关(P<0.05),淋巴结转移组中其阳性表达率明显低于无淋巴结转移组(P<0.01),与肿瘤分化程度、年龄无关(P>0.05)。③Survivin在宫颈癌中的阳性表达与临床分期、病灶大小和肿瘤分化程度有关(P<0.05),淋巴结转移组中其阳性表达率明显高于无淋巴结转移组(P<0.01),而与年龄大小无关(P>0.05)。④PTEN和Survivin在宫颈上皮内瘤变和宫颈癌中的阳性表达呈负相关。结论:抑癌基因PTEN、凋亡抑制基因Survivin在宫颈癌变过程中协同参与了宫颈癌的发生和进展。PTEN和Survivin在宫颈癌组织中阳性表达为其早期诊断和预后判断提供了新的评价指标,也为宫颈癌的基因治疗提供了新的生物学靶点。Objective ： To evaluate the expression of FFEN and Survivin in the successive steps of progression in cervical neoplasia and to determine their correlation with clinicopathologic features in squamous cell carcinoma of the uterine cervix. Methods： Immunohisto- chemical staining with anti - PTEN and anti - Survivin antibody were performed in a total of 109 patients with 10 normal cervical epithelium （as control） , 20 cervical erosion metaplasia, 30 cervical intraepithelial neoplasia, and 49 cervical squamous cell carcinomas. Results： ① PTEN expression progressively decreased along the continuum from normal epithelium to squamous cell carcinoma （P 〈 0. 05） . In contrast , Survivin expression progressively increased （ P 〈 0. 05 ） . ②Positive FFEN immunostaining was associated with clinical stage （ P 〈 O. 05 ）, tumor size （P 〈 0. 05） and metastatic pelvic lymph nodes （P 〈 0. O1 ） . No significant differences with the expression of PTEN were found in relation to the and the d：.fferentiated degree （P 〉 0. 05 ） . ③Positive Survivin immunostaining was associated with clinical stage （ P 〈 0. 05）, tumor size （ P 〈 0. 05）, differentiated degree （P 〈 0. 05 ）, and metastatic pelvic lymph nodes （P 〈 0. 01 ） . No obvious relation- ship between Survivin expression and the patient＇s age was found （P 〉 0. 05 ） . ④The expression of PTEN and Survivin were inversely corre- lated in cervical intraepithelial neoplasia and cervical squamous cell carcinoma （P 〈0.0l） . Conclusion： PTEN and Survivin synergistical- ly involved in the development and progression of cervical cancer. Positive expression of PTEN and Survivin may be two valuable biomarkers that are predictive of the progression and prognosis in patients with cervical squamous cell carcinoma, may also have potential as promising therapeutic targets in gene therapy of cervical cancer.

关 键 词：PTEN SURVIVIN 宫颈上皮内瘤 宫颈鳞状上皮细胞癌 肿瘤标志物 

分 类 号：R737.33[医药卫生—肿瘤]