罗格列酮对肝星状细胞基质金属蛋白酶抑制因子1、2基因表达的影响  

Effects of rosiglitazone on expression of tissue inhibitor of metalloproteinase-1 and tissue inhibitor of metalloproteinase-2 in hepatic stellate cells

作　　者：付晓霞[1] 王爱民[1] 张莉[1] 周绵[1] 李洵[1] 

机构地区：[1]解放军252医院消化科,河北保定071000

出　　处：《中华实验外科杂志》2013年第9期1893-1894,共2页Chinese Journal of Experimental Surgery

摘　　要：目的 观察过氧化物酶体增殖物活化受体γ（PPARγ）特异配体罗格列酮对肝星状细胞（HSC）表达基质金属蛋白酶抑制因子1（TIMP-1）、TIMP-2的影响.方法 在培养的HSC株中加入不同浓度的罗格列酮（终质量浓度为5、10、15、20 μmol/L）,于24h后收集细胞,利用半定量逆转录-聚合酶链反应（RT-PCR）测定TIMP-1、TIMP-2 mRNA的表达水平.结果 TIMP-1 mRNA表达水平在罗格列酮5、10、15、20 μmol/L组分别为1.8087±0.0642、1.3517±0.0485、0.9510±0.0835、0.5653±0.0879;而空白对照组为2.0508±0.1576.TIMP-2 mRNA表达水平在罗格列酮5、10、15、20 l,mol/L组分别为3.1314±0.0717、2.0758±0.0730、1.4718±0.0623、0.8566±0.0744;而空白对照组为3.5784±0.0956.结论 PPARγ特异配体罗格列酮可抑制HSC表达TIMP-1、TIMP-2,并在一定范围内呈剂量依赖性.Objective To investigate the effects of rosiglitazone,a ligand of peroxisome proliferators activated receptor gamma （PPAR-γ）,on the expression of tissue inhibitor of metalloproteinase （TIMP）-1 and TIMP-2 in hepatic stellate cells （HSCs）.Methods HSCs were cultured in medium containing different concentrations of rosiglitazone （5,10,15,20 μmol/L）,and harvested at 24 h.The expression of TIMP-1 and TIMP-2 was detected by using reverse transcription polymerase chain reaction.Results TIMP-1 gene expression levels in HSCs cultured with different concentrations of rosiglitazone （5,10,15,20 μmoL/L） were 1.8087 ± 0.0642,1.3517 ± 0.0485,0.9510 ± 0.0835 and 0.5653 ± 0.0879 respectively,and it in control group was 2.0508 ±0.1576.The expression of TIMP-2 in HSCs cultured with different concentrations of rosiglitazone （5,10,15,20 μmoL/L） was 3.1314 ±0.0717,2.0758 ±0.0730,1.4718±0.0623 and 0.8566 ±0.0744 respectively,and it in control group was 3.5784 ±0.0956.Conclusion PPAR-γ ligand rosiglitazone may down-regulate the expression of TIMP-1 and TIMP-2 in HSC dose-dependently to some extent.

关 键 词：肝星状细胞 氧化物酶体增殖物活化受体γ 罗格列酮 基质金属蛋白酶抑制因子 

分 类 号：R575.2[医药卫生—消化系统]