微小RNA-301a介导高糖促进前列腺癌细胞周期G1/S时相转换  

MicroRNA-301a mediates cell cycle G1/S phase transition promoted by high glucose in prostate cancer cells

作　　者：蔡燚[1] 李小娟[2] 朱宝益[1] 陶奕然[1] 李骏[1] 蔡松旺[3] 陈锐涵[4] 温星桥[1] 

机构地区：[1]中山大学附属第三医院泌尿外科,广州510630 [2]中山大学附属第三医院保健科,广州510630 [3]中山大学附属第三医院心胸外科,广州510630 [4]中山大学附属第三医院急诊外科,广州510630

出　　处：《中华实验外科杂志》2013年第9期1927-1929,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81072115、30901768）;中山大学985创新团队项目（82000-3281901）

摘　　要：目的 探讨微小RNA（miR）-301a介导高糖促进前列腺癌细胞生长的分子机制.方法 通过实时定量聚合酶链反应（Real-time PCR）检测miR-301a的表达.细胞计数试剂盒（CCK-8）法检测转染miRNAs和/或高糖（4.5 g/L）培养的前列腺癌DU145细胞的增殖.应用微管聚合抑制剂诺考达唑（Nocodazole,40 μg/L）处理16h或者血清饥饿处理48 h使细胞周期同步化,流式细胞仪分析miR-301a对细胞周期时相转换的影响.CCK-8法测定细胞生长活性,检测高糖及miR-301a对前列腺癌细胞增殖的影响.结果 miR-301a在前列腺癌细胞PC3、DU145的表达分别为正常前列腺上皮细胞RWPE-1的（2.86±0.75）倍和（4.05±0.96）倍.高糖培养DU145细胞24、48 h后miR-301a的表达分别为对照组的（1.88±0.34）倍和（13.15 ±3.45）倍.抑制miR-301a可部分逆转高糖的促增殖作用.过表达miR-301a后经细胞周期同步化处理,与对照组比较miR-301a可促进前列腺癌DU145细胞G1/S时相转换.结论 miR-301a在前列腺癌细胞中表达上调,miR-301a介导高糖促进前列腺细胞周期G1/S时相转换,促进细胞增殖.Objective To investigate the molecular mechanism of microRNA （miR）-301a in the regulation prostate cancer cell proliferation by high glucose.Methods The expression of miR-301a was detected by real-time quantitative polymerase chain reaction （Real-time PCR） （Real-time PCR）.cell counting kit-8 （CCK-8） assay was performed to evaluate the influence of high glucose （4.5 g/L） and/or miRNAs transfection on the proliferation of DU145.Nocodazole-treated for 16 hours or serum-starved for 48 hours were used to synchronized cell cycle,the transition of cell cycle was detected by flow cytometry.Results The expression level of miR-301a in PC3 and DU145 cells were 2.86 ±0.75 and 4.05 ±0.96 times higher than the RWPE-1 cells respectively.The expression of miR-301a were 1.88 ± 0.34 and 13.15 ±3.45 times higher than control group in DU145 cells cultured in high glucose for 24 and 48 hour.Inhibition of miR-301 a could reverse the effect on proliferation of high glucose.Overexpression of miR-301 a promoted G1/S phase transition in prostate cancer DU145 cells.Conclusion miR-301a was up-reglulated in prostate cancer cell lines,which mediates G1/S cell cycle phase transition promoted by high glucose.

关 键 词：高糖 微小RNA-301a 前列腺癌 细胞周期 

分 类 号：R737.25[医药卫生—肿瘤]