伊洛马司他对人喉癌Hep-2细胞增殖及凋亡的影响  

Effects of ilomastat on the proliferation and apoptosis of human laryngeal cancer Hep-2 cells

作　　者：李黎[1] 高树峰[1] 张少容[1] 刘月辉[1] 兰宁[1] 徐莲[1] 

机构地区：[1]南昌大学第二附属医院耳鼻咽喉头颈外科,330006

出　　处：《实用医学杂志》2013年第19期3116-3119,共4页The Journal of Practical Medicine

基　　金：江西省教育厅科学技术研究项目(编号:GJJ11361)

摘　　要：目的:探讨基质金属蛋白酶抑制剂伊洛马司他对人喉癌Hep-2细胞增殖活性及凋亡水平的影响,为喉癌的临床用药提供参考。方法:应用MTT法分析不同浓度伊洛马司他对Hep-2细胞增殖活性的影响,并计算该药的IC10及IC50;应用流式细胞术检测接近IC10浓度伊洛马司他作用于Hep-2细胞不同时间后的凋亡率。结果:MTT结果示不同浓度伊洛马司他对Hep-2细胞均有抑制作用,且抑制率呈浓度依赖性(P<0.05),此药的IC10及IC50分别为8和130μg/mL(P<0.05);流式细胞术结果示伊洛马司他可诱导Hep-2细胞的凋亡,且凋亡率依赖于药物作用时间的增加(P<0.05)。结论:伊洛马司他可抑制Hep-2细胞的增殖,诱导其凋亡,从而抑制喉癌的发生与发展,为喉癌的临床用药提供了一定的实验依据。Objective To explore the effects of ilomastat on the proliferation and apoptosis of human laryngeal cancer Hep-2 cells. Methods The proliferation activity of the Hep-2 cells treated with different concentrations of ilomastat was analyzed by MTT assay, and the IC10 and IC50 of ilomastat were calculated. Flow cytometry was used to detect the apoptosis rate of Hep-2 cells that were given IC10 concentration of ilomastat in different times. Results Ilomastat could inhibit the proliferation of Hep-2 cells in a dose-dependent manner （P 〈 0.05）, and the IC10 and IC50 values of ilomastat were 8 μg/mL and 130μg/mL respectively. And also ilomastat could induce the apoptosis of Hep-2 cells in a time-dependent manner （P 〈 0.05）. Conclusion Ilomastat can inhibit the proliferation and can induce the apoptosis of Hep-2 ceils, and finally inhibit the progression of laryngeal cancer.

关 键 词：喉肿瘤 伊洛马司他 增殖 凋亡 

分 类 号：R914[医药卫生—药物化学]