反义P-PNA对SPC-A-1凋亡作用的表达  

The expression of antisense P-PNA on apoptosis of SPC-A-1

作　　者：谷勇[1] 胡东霞[2] 胡晖 

机构地区：[1]江西省胸科医院,南昌330006 [2]南昌大学第二附属医院康复医学科 [3]江西省宜春市奉新县人民医院,330700

出　　处：《中国医疗前沿》2013年第15期22-22,28,共2页China Healthcare Innovation

摘　　要：目的探讨反义肽核酸(phosphono sense peptide nucleic acid,P-PNA)对肺癌SPC-A-1凋亡作用的影响。方法采用肺癌细胞株(SPC-A-1)培养,待细胞为对数生长期时,传代培养30例标本,分为P-PNA组、脂质体组、空白对照组,每组10例,转染前后用RT-PCR,流式细胞仪检测,分别检测端粒酶活性和细胞凋亡百分率。结果转染后P-PNA的端粒酶活性水平有所下降,ATP的浓度明显降低,空白对照组与脂质体组无明显的变化(P<0.05)。P-PNA组肺癌细胞的凋亡率明显增加,与其他两组比较差异显著(P<0.05)。结论 P-PNA在载体运载下,可以进入SPC-A-1细胞线粒体中,通过影响端粒酶的活性阻碍编码基因的转录,进而影响肺癌细胞的合成并诱导其凋亡。Objective To investigate the effect of antisense peptide nucleic acid（phosphono sense peptide nucleic acid, P-PNA） on lung cancer SPC-A-1 apoptosis. Methods The lung cancer cells line（SPC-A-1） were cultured, logarithmic growth phase, 30 specimens were taken for subculture. They were divided into the P-PNA group, the liposome group, the blank control group, and 10cases in each group. The RT-PCR, flow cytometry was taken for detection before and after transfection. The percentage of telomerase activity and apoptosis was taken for detection. Results The level of P-PNA after transfection of telomerase activity decreased, the concentration of ATP decreased significantly, the blank control group and liposome group had no significant changes（P〈0.05）. Group P-PNA lung cancer cell apoptosis rate increased significantly, and there were significant differences compared with the other two groups（P〈0.05）. Conclusion P-PNA in the carrier vehicle, can enter the mitochondria of SPC-A-1 cells by telomerase activity, impede the transcription of genes encoding, and affect the synthesis of lung cancer cells and induce its apoptosis.

关 键 词：肺癌 反义P-PNA 端粒酶 转录 凋亡 

分 类 号：R364.2[医药卫生—病理学]