siRNA靶向沉默OCT4基因对前列腺癌DU145和PC3细胞等生物学行为的影响  

Role of Oct4 RNAi on influence of prostate cancer DU145 and PC3 cell

作　　者：庄岩[1] 胡亮亮[1] 孙晓青[1] 

机构地区：[1]徐州医学院,江苏221002

出　　处：《国际泌尿系统杂志》2013年第5期577-581,共5页International Journal of Urology and Nephrology

摘　　要：目的 观察siRNA靶向沉默OCT4基因对前列腺癌DU145细胞和PC3细胞的生物学行为影响.方法 通过脂质体介导的方法将OCT4 SiRNA分别转染人前列腺癌细胞株DU145细胞和PC3细胞,采用RT-PCR和Western-blot方法分别检测特异性siRNA对0ct4基因在mRNA和蛋白水平上的沉默效果,CCK8法测定细胞生长曲线观察细胞增殖的抑制情况,运用Transwell小室法检测细胞侵袭能力的变化.结果 转染OCT4 SiRNA的两组细胞OCT4的表达均低于阴性对照组（NC）与空白组（P＜0.05）,OCT4SiRNA抑制两种细胞的增殖并降低细胞的侵袭能力.结论 OCT4-siRNA可以有效抑制DU45细胞和PC3细胞的OCT4基因的表达,从而抑制细胞增殖,抑制侵袭和迁移,表明OCT4基因在前列腺癌的发生发展中发挥重要作用.Objectives To investigate the effects of silencing of OCT4 gene expression by siRNA on the biological behavior of human prostate cancer DUl45 cells and PC3 cells.Methods The siRNA targeting 0ct4 was transfected into DU145 cells and PC3 cells by Lipofectamine 2000（Lipo）,The expression levels of OCT4 was examined by RT-PCR and Western boltting.The change of proliferation was detected by CCK-8.The activities of motility and invasion were assessed by transwell chamber invasion assay in vitro.Results The expression levels of OCT4 mRNA and protein in the cells transfected with siRNA were significantly lower than in blank group and in negative control（NC） group （P ＜0.05）.The proliferation of DU145 cells and PC3 cells was significantly inhibited and the invasion of the two cell groups was decreased after transfected by OCT4 siRNA（P ＜0.05）.Conclusions The inhibition of OCT4 expression by siRNA could inhibit cell proliferation and decrease the invasion in DUl45 cells and PC3 cells which suggest that OCT4 play a vital role in the gliomagenesis and development of prostate cancer.

关 键 词：前列腺肿瘤 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]