美洲大蠊提取物抗肝损伤药效比较及工艺评价  

Comparison of Effects Against Hepatic Injury among Americana Linn Extracts and Evaluation of Extraction Processes

作　　者：黄晶[1] 梅明[1] 许润春[1] 熊永爱[1] 李晨晨[1] 杨明[1,2] 

机构地区：[1]成都中医药大学,成都611137 [2]江西中医学院中药现代制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2013年第19期46-49,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:比较不同工艺制备的美洲大蠊提取物抗肝损伤药效与主要成分含量差异。方法:采用水或乙醇分别回流法、闪式法和磁力搅拌法提取美洲大蠊,通过CCl4所致小鼠急性肝损伤模型考察药效,采用考马斯亮蓝法和茚三酮法测定美洲大蠊提取物中总肽及游离氨基酸含量,结合出膏率计算加权得分,考察不同提取工艺的差异。结果:水闪式提取物具有良好的对抗CCl4所致急性肝损伤小鼠血清MDA增多、抑制血清转氨酶增高、减轻肝组织病变的作用。各提取物中出膏率分别为16.64%,20.84%,16.19%,15.75%,19.53%,16.82%,总肽质量分数分别为0.82%,0.03%,0.96%,0.02%,0.64%,0.02%,游离氨基酸质量分数分别为1.99%,1.11%,2.12%,1.15%,2.10%,1.03%,综合评分分别为90.64,37.31,99.46,42.53,82.42,39.00。结论:美洲大蠊水闪式提取物具有良好的抗肝损伤药效与工艺指标评分。Objective： To compare differences of efficacy against hepatic injury and contents of major ingredients among Americana Linn extracts prepared by different processes. Method： With water and ethanol as solvents, Americana Linn was extracted by reflux method, smashing method and magnetic stirring method, respectively. Efficacy was tested with acute hepar-injured mice model caused by CCl4, the contents of total peptides and free-amino acid were determined by Bradford and Ninhydrin colorimetric method, combining with cream rate, differences among processes was investigated by calculating weighted score. Result： Water smashing- extract could obviously resist increasing of MDA, ALT and AST in serum and relieve pathological changes of acute hepar-injured mice caused by CC14. In each extracts, cream rates were 16.64% , 20.84% , 16. 19% , 15.75% , 19.53% and 16.82% , respectively; The contents of total peptides were 0. 82% , 0.03% , 0.96% , 0.02% , 0.64% , 0.02% , respectively; The contents of free amino acid were 1.99% , 1.11% , 2.12% , 1.15% , 2.10%, 1.03%, respectively; Composite scores were 90.64, 37.31, 99.46, 42.53, 82.42, 39.00, respectively. Conclusion： Water smashing-extract of Americana Linn had a good efficacy against hepatic injury . and a high score of technic indexes.

关 键 词：美洲大蠊 闪式提取法 总肽 游离氨基酸 肝损伤 

分 类 号：R283.6[医药卫生—中药学] R285.5[医药卫生—中医学]