红树共附生棘孢曲霉CGD12的次级代谢产物及其抑菌活性  

Secondary Metabolites of Aspergillus aculeatus CGD12 Isolated from Mangrove and Their Antibacterial Activities

作　　者：李英新[1] 焦伟华[2] 周兴春[1] 张俊[1] 叶波平[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009 [2]第二军医大学长征医院药学部,上海200438

出　　处：《中国实验方剂学杂志》2013年第19期71-76,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072571)

摘　　要：目的:分离一株红树来源的棘孢曲霉CGD12的次级代谢产物并检测它们的抑菌活性。方法:采用正反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备高效液相色谱等方法对菌株发酵产物的乙酸乙酯萃取部分进行分离和纯化,利用NMR,MS等波谱分析方法确定化合物的结构;利用微量肉汤稀释法进行抑菌活性评价。结果:从菌株发酵液乙酸乙酯提取物中分离鉴定了7个化合物,分别为:versiol(1)、JBIR74(2)、JBIR75(3)、环(L-脯氨酸-L-酪氨酸)(4)、环(L-脯氨酸-L-缬氨酸)(5)、环(L-脯氨酸-L-异亮氨酸)(6)、环(L-脯氨酸-L-亮氨酸)(7),其中化合物4,7对金黄色葡萄球菌(S.aureus)和表面葡萄球菌(Staphyloccocus epidermidis)的生长显示出一定的抑制活性,而化合物3对绿脓杆菌(Pseudomonas aeruginosa)、肺炎克雷伯菌(Klebsiellar pneumonia)和大肠埃希菌(Escherichia coli)具有较弱的抑菌活性。结论:从棘孢曲霉菌株CGD12中分离到7个已知的化合物,其中化合物3,4和7分别具有一定的抑菌活性。Objective： The secondary metabolites of marine fungus Aspergillus aculeatus CGD12 and their antibacterial activities were investigated. Method： Column chromatography on silica gel, ODS, Sephadex LH-20 and semipreparative HPLC were used to isolate and purify the compounds from EtOAc extract of the fermentation broth. Their structures were elucidated by NMR, MS spectroscopics data analyses and compared with the data of reported literatures. In order to evaluate the antibacterial activities of these compounds, microdilution broth method was adopted. Result： Seven compounds were isolated from the fermentation broth of A. aculeatus CGD12 and identified as versiol （1）, JBIR74 （2）, JBIR75 （3）, cyclo- （L-Pro-L-Tyr） （4）, cyclo- （L-Pro-L.Val） （S）, cyclo- （L-Pro-L-Ile） （6）, cyclo- （L-Ala-L-Leu） （7）. Conclusion： Compounds 4 and 7 show antibacterial activities against Staphyloccocus aureus and S. epidermidis. The weak antibacterial activities which against Pseudomonas aeruginosa, Klebsiellar pneumonia and Escherichia coil of compound 3 are reported for the first time.

关 键 词：红树 棘孢曲霉 代谢产物 抑菌活性 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]