姜黄素类似物诱导A549细胞自噬和抑制增殖的作用  

Curcumin analogs induce autophagy in A549 cells and inhibit proliferation

作　　者：徐亚来[1] 周广舟[2] 夏熙郑[1] 陈小兵[2] 

机构地区：[1]郑州大学第二附属医院呼吸内科,郑州450008 [2]河南工业大学生物工程学院,郑州450008

出　　处：《中国癌症防治杂志》2013年第3期216-221,共6页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

摘　　要：目的初步探究姜黄素类似物Sunwsz08诱导人非小细胞肺癌(A549)细胞自噬现象和抑制增殖的作用。方法选用不同浓度的姜黄素类似物Sunwsz08作用于体外培养的A549细胞中,于倒置显微镜下观察细胞形态;吖啶橙、PI染色药物作用后在荧光显微镜下观察A549细胞内酸性颗粒及细胞死亡情况;MTT法检测Sunwsz08对A549细胞增殖的抑制作用;GFP-LC3自噬定位法观察细胞自噬泡。结果姜黄素类似物Sunwsz08在16μmol/L、20μmol/L及更高浓度时,A549细胞空泡增加,细胞形态明显异常,细胞死亡现象常见。Sunwsz08在8μmol/L、10μmol/L浓度时,吖啶橙染色可见明显酸性小泡;PI染色发现随着药物浓度增加及作用时间的延长,A549细胞死亡现象增加。MTT法检测示姜黄素类似物Sunwsz08对A549细胞增殖的抑制作用明显,其抑制效果随着药物浓度增加和时间的延长而增强。结论姜黄素类似物Sunwsz08可诱导A549细胞自噬并且对细胞增殖有抑制作用。Objective To explore the ability of eureumin analog Sunwsz08 to induce autophagy in A549 cells as well as inhibit their proliferation. Methods A549 cultures were incubated with various concentrations of Sunwsz08 for different durations, and their mor- phology was examined using an inverted microscope.The cultures were stained with acridine orange and propidium iodide, and their morphology was further examined by fluorescence microscopy.In order to examine the ability of Sunwsz08 to promote autophagy, GFP- LC3 positioning was used to identify autophagic vacuoles.The MTT assay was used to assess the compound＇s ability to inhibit cellular proliferation. Results At concentrations of 8 and 10 mmol/L,Sunwsz08 significantly increased autophagy in A549 cells.At concentra- tions above 16 mmol/L,the analog caused an increase in the number of cell vacuoles, and it induced abnormal cell morphology and cell death.Sunwsz08 also inhibited A549 proliferation in a dose-and time-dependent manner. Conclusion The curcumin analogue Sunwsz08 can induce autophagy and inhibit proliferation in A549 ceils.

关 键 词：肺肿瘤 姜黄素类似物 A549细胞 自噬 增殖 抑制 

分 类 号：R734.2[医药卫生—肿瘤]