Kaposi肉瘤组织人类疱疹病毒8型免疫组化检测  

Detection of human herpesvirus 8 in Kaposi's sarcoma by immunohistochemistry

作　　者：李淑艳[1] 张德志[1] 刘勇[1] 靳颖[1] 普雄明[1] 

机构地区：[1]新疆维吾尔自治区人民医院皮肤性病科,乌鲁木齐830001

出　　处：《实用皮肤病学杂志》2013年第5期266-268,共3页Journal of Practical Dermatology

基　　金：新疆维吾尔自治区自然科学基金资助项目(2009211A24);国家临床重点专科建设项目经费资助

摘　　要：目的探讨免疫组化检测Kaposi肉瘤(KS)组织人类疱疹病毒8型(HHV-8)的可行性及其诊断意义。方法采用免疫组化方法分别检测58例KS组织和40例化脓性肉芽肿组织中HHV-8的表达。结果①HHV-8在KS真皮瘤体中的表达阳性率为94.83%(55/58),在化脓性肉芽肿表达均阴性,在KS组织和血管瘤组织瘤体中的表达差异有统计学意义(P<0.05)。②HHV-8在KS组织表皮中表达阳性率为6.90%(4/58),血管内皮细胞中的表达阳性率为91.38%(53/58),真皮梭形细胞中表达阳性率为94.83%(55/58)。③结节期KS组织中表达HHV-8阳性的细胞所占比例较斑片期及斑块期明显增多,其差异有统计学意义(P<0.05)。结论免疫组化方法检测HHV-8在KS的诊断中具有重要意义。Objective To explore the feasibility and diagnostic significance of immunohistochemistry in detecting of human herpesvirus 8（HHV-8） in Kaposi＇s sarcoma（KS）. Methods The expression of HHV-8 was detected by immunohistochemistry in 58 cases of KS and 40 cases of pyogenic granuloma tissue. Results Among 58 cases of KS in the dermis, 55 cases were positive （94.83%）. Among 40 cases of pyogenic granuloma, none was positive. The difference of HHV-8 expression between KS and pyogenic granuloma had statistical significance （P〈0.05）. The expression of HHV-8 in the epidermis of KS, 4 cases were positive（6.90%）. 53 cases were positive （91.38%） in the vascular endothelial cell of KS. 55 cases were positive （94.83%） in the spindle cell of the dermis of KS. The proportion of positive cells were higher in the nodules stage of KS than patch stage and plaque stage. The difference of the positive cell proportion in the different stages of KS had statistical significance （P〈0.05）. Conclusion The immunohistochemical detection of HHV-8 in KS has diagnostic significance.

关 键 词：肉瘤 Kaposi 人类疱疹病毒8型 免疫组化检测 

分 类 号：R730.2[医药卫生—肿瘤]