微藻氮源对红薯原料燃料乙醇发酵过程促进效果的研究  

FERMENTATION PROMOTION BY ALGAL NITROGEN SOURCE FEEDING IN FUEL ETHANOL PRODUCTION FROM SWEET POTATO MASH

作　　者：申渝[1,2,3] 张海东[1,2] 郭劲松[3] 陈猷鹏[3] 郑旭煦[2] 张贤明[1] 

机构地区：[1]废油资源化技术与装备教育部工程研究中心,重庆工商大学,重庆400067 [2]催化与功能有机分子重庆市重点实验室,重庆工商大学,重庆400067 [3]重庆绿色智能技术研究院,中国科学院,重庆401122

出　　处：《太阳能学报》2013年第9期1542-1546,共5页Acta Energiae Solaris Sinica

基　　金：国家重大科技专项(2009ZX07104-001);重庆市教委自然科学基金(KJ130702;KJ130729;KJZH11212);重庆市科委自然科学基金(CSTC2012jjB0138)

摘　　要：红薯干粉碎后经双酶法处理得到葡萄糖含量为210.0g/L的醪液,利用工业酿酒酵母Saccharomyces cerevise ATCC 6508批式发酵产乙醇。野生水华微藻生物质收集后,经洗涤、稀盐酸水解、干燥、粉碎制得微藻氮源(Algal Nitrogen Source,ANS)。实验发现:在未添加任何氮源条件下发酵120h时乙醇浓度为89.14g/L,残余葡萄糖为7.0g/L,发酵葡萄糖的乙醇得率为0.44;添加ANS对发酵过程的促进效果显著,当ANS添加量分别为5.0、10.0和20.0g/L时,发酵终点时间(残余葡萄糖<1.0g/L)分别为96、72和72h,终点乙醇浓度分别为96.1、98.1和103.0g/L,乙醇得率分别为0.46、0.47和0.49。蛋白质和氨基酸含量分析表明,ANS和酵母粉蛋白质含量为32.6%和64.5%(干重),游离氨基酸含量为19.7%和29.9%(干重),高含量的有机氮源,特别是游离氨基酸,对发酵效率和乙醇得率的提高有重要作用。对比实验发现,添加10.0g/L ANS添加3.0g/L酵母粉的发酵参数相当,因此ANS可替代酵母粉作为红薯原料乙醇发酵的氮源添加剂。Sweet potato medium contained 210. 0g/L glucose was produced from sweet potato powder based two enzyme method, and was used for fuel ethanol fermentation by Saccharomyces cerevise ATCC 6508. Bloom algae biomass was collected and processed into algal nitrogen source （ANS） after processing steps including wash, acid hydrolysis, drying and milling. It was indicated that the residue glucose was 7.0g/L in batch without nitrogen source feeding after 120h fermentation, and the yield of ethanol and final ethanol concentration were 0. 44 and 89. 14g/L. While the fermentation times were reduced to 96, 72 and 72h when 5.0, 10. 0 and 20. 0g/L ANS was added, 96. 1,98. 1 and 103.0g/L final ethanol concentrations were attained, ethanol yields amounted to 0. 46, 047 and 0. 49 respectively. Protein and amino acid analysis proved that the protein content in ANS and yeast extract were 32. 6 and 64. 5%, and free amino acid content were 19. 7 and 29. 9%, high content of organic nitrogen matter was expected to be responsible for fermentation rate promotion. The study also indicated ANS was a reliable alternative nitrogen source for yeast extract in ethanol fermentation using sweet potato mash as the medium.

关 键 词：红薯原料 微藻氮源 燃料乙醇发酵 发酵效率 乙醇得率 

分 类 号：TK6[动力工程及工程热物理—生物能]