款冬花多糖硫酸化修饰条件的优化  

Optimization of the sulfated modification of polysaccharides fromTussilago farfara

作　　者：张琳[1] 张善文[1] 赵鹏[1] 

机构地区：[1]陕西中医学院药学院,咸阳712046

出　　处：《西北药学杂志》2013年第5期475-478,共4页Northwest Pharmaceutical Journal

基　　金：陕西省自然科学基金资助项目(编号:2011JQ2005);陕西省教育厅自然科研专项基金(编号:2013JK0764)

摘　　要：目的对款冬花多糖(TFPs)进行硫酸酯化修饰最优化工艺的研究,并初步探讨其抗氧化活性。方法采用氯磺酸-吡啶法,制备款冬花硫酸酯化多糖(TFPsS);以取代度为指标,采用响应面法对制备工艺条件进行优化;并进行TFPsS清除自由基DPPH·实验,研究其体外抗氧化活性。结果最佳酯化条件为:酯化试剂体积比为3.6,反应温度为95℃,时间为2.4h。TFPsS具有较好的清除能力;与TFPs相比,清除羟自由基的能力显著提高。结论氯磺酸-吡啶法适用于款冬花多糖的硫酸酯化修饰,对其进行硫酸酯化修饰有助于提高抗氧化能力。Objective To optimize the sulfated modification conditions of Tussilago farfara polysaccharides（TFPs）and to study the antioxidant activity.Methods Polysaccharides from Tussilago farfara was sulfated by chlorosulfonic acid-pyridine method.According to the degree of substitution,response surface methodology was adopted to optimize the sulfated modification process.The effects of sulfated Tussilago farfara polysaccharides（TFPsS）on scavenging DPPH·was determined.Results The result showed that 3.6volume ratio of pyridine and chlorosulfonic acid,95 ℃,2.4hwere the optimal conditions.Compared with TFPs,the effect of TFPsS on scavenging DPPH·was improved.Conclusion The chlorosulfonic acid-pyridine method was effective and TFPsS of scavenging DPPH·was improved.

关 键 词：款冬花 多糖 硫酸酯化 抗氧化性 

分 类 号：R284[医药卫生—中药学]