固定离子液体/硅溶胶/壳聚糖复合膜中联吡啶钌电化学发光行为的研究及应用  

Electrochemiluminescence based on an ionic liquid /silica sol /CS-Tris( 2,2'-bipyridine) ruthenium( Ⅱ)film modified gold electrode and its analytical application

作　　者：杨兰兰[1] 李利军[1] 张瑞瑞[1] 罗应[1] 崔福海[1] 崔越[1] 孙科[1] 李彦青[1] 

机构地区：[1]广西科技大学生物与化学工程学院,柳州545006

出　　处：《分析试验室》2013年第10期1-5,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31060219)资助

摘　　要：利用离子液体(Ionic liquid)的独特性质,以硅溶胶(Silica sol)为主要载体,将联吡啶钌固定到金电极上,成功制备了一种新型的电化学发光传感器。并采用循环伏安(CV)和电致化学发光(ECL)法,考察了联吡啶钌和盐酸硫必利在此修饰电极上的电化学(EC)及其发光行为。发现盐酸硫必利对固定化联吡啶钌在0.1 mol/L的磷酸盐缓冲液(PBS)介质中的弱电化学发光信号有较强的增敏作用,建立了盐酸硫必利的电化学发光新方法。在最佳实验条件下,盐酸硫必利浓度在4.0×10-7～1.0×10-4mol/L范围内与相对发光强度呈线性关系(r2=0.9962),检出限(S/N=3)为1.82×10-9mol/L。连续平行测定1.0×10-5mol/L的盐酸硫必利溶液10次,发光强度的RSD为1.6%。对样品进行回收率试验,回收率在96.7%～107.7%之间。该方法用于样品盐酸硫必利的测定,结果满意。A novel electrochemiluminescence sensor was fabricated by employing the nature of ionic liquid and silica sol as the carrier and tris（2,2＇-bipyridyl） ruthenium（Ⅱ） [Ru（bpy） 3 2 ＋]were immobilized on a gold electrode.The electrochemical and ECL characteristics of Ru（bpy） 3 2 ＋and Levofloxacin at the modified electrode were studied,and the modified electrode exhibited good electrochemical activity and ECL response.Based on the obvious electrochemiluminescence sensitization effect of tiapride hydrochloride with Ru（bpy） 3 2 ＋in the 0.1 mol / L phosphate buffer,a new electrochemiluminescence method for the determination of tiapride hydrochloride was developed.Under the optimum conditions,the calibration curve showed a good linearity with tiapride hydrochloride concentration in the range of 4.0 × 10-7～ 1.0 × 10-4mol / L（r2= 0.9962） and the detection limit（S / N = 3） was 1.82 × 10-9mol / L.The RSD for 10-time determination of 1.0 × 10-5mol / L tiapride hydrochloride was 1.6 %（n = 10）.The results of recovery test indicated that the recoveries were 96.7% ～ 107.7%.This method was applied to the analysis of tiapride hydrochloride tablets with satisfactory results.

关 键 词：电化学发光 联吡啶钌 硅溶胶 离子液体 壳聚糖 

分 类 号：O657.1[理学—分析化学]