从山茶花花卉中提取多酚的方法和工艺研究  

Methods and Processes for Extracting Polyphnols from the Flowers of Camellia japonica

作　　者：邓骛远[1] 邹家丽[2] 和七一[2] 赵瑛[2] 邓可宣[2] 张近春[2] 杨宪[2] 余晓东[2] 罗通[1] 

机构地区：[1]宜宾学院生物研究所,四川宜宾644000 [2]重庆师范大学生命科学学院重庆市动物生物学重点实验室重庆市生物活性物质工程研究中心,重庆401331

出　　处：《重庆师范大学学报（自然科学版）》2013年第5期114-119,共6页Journal of Chongqing Normal University:Natural Science

基　　金：宜宾市科技计划项目(No.2011Z27/2012);宜宾学院科研基金项目(No.2012S13/2012)

摘　　要：为了从山茶花卉中浸提并沉淀得多酚,采用单因素实验和正交实验(包括温度、时间、料液比、乙醇浓度等)得出最佳的浸提条件;然后采用金属离子沉淀方法从该浸提液中沉淀得到多酚物质,考察了金属离子、pH值、不同类型和浓度碱性溶液对沉淀率的影响。结果表明,1)浸提的最佳条件应为:浸提溶液为60%乙醇,浸提温度为80℃,浸提时间为20min,浸提的料液比为1∶35;2)发现多种金属离子沉淀剂(Al 3+、Fe3+、Zn2+、Mg2+、Ba2+和Ca2+)中Al 3+的沉淀效果最好,且沉淀的最佳pH值为5.5,所得多酚的最终收率为7.1%,纯度为72.47%。研究认为上述方法提取的多酚纯度较好,下一步需扩展研究规模以指导生产应用。Extracting and precipitating polyphenol from the Camellia japonica flowers. Using single factor experiments and orthog- onal experiments to study the optimal conditions （including temperature, time, ratio of solid-to-liquid, ethanol concentration etc ） ; and then using the method of metal ion precipitation to extract polyphenols from the extract liquid, and in the process we studied the influence of metal ion, pH, several alkaline solutions and their concentration on sedimentation rate （methods）. The best conditions are as follows： 60% of ethanol concentration, 80 ℃ for temperature, the time for 20 min, and 1：35 of the solid-liquid ratio; a- mong various precipitating agents of metal ions （AP＋ , Fe3＋ , Zn2＋ , Mg2＋ , Ba2＋ and Ca2＋ ）. Al3＋ presents the best precipitation effect, and its optimal pH for precipitation is 5.5. Finally, the final yield of obtaining polyphenols is 7. 1% and its purity is 72.47 %. Purity of polyphenols is well in this methods, futher research is needed to have large-scale production.

关 键 词：多酚 金属离子沉淀 浸提 山茶花 

分 类 号：Q586[生物学—生物化学]