microRNA-1Ob促进人低转移肺癌细胞株95-C的增殖及侵袭  

miRNA-10b promote proliferation and invasion of low metastasis of lung cancer cell line 95-C

作　　者：刘翼[1] 李明慧[1] 郭文佳 张国庆[1] 庞作良[1] 

机构地区：[1]830011乌鲁木齐,新疆医科大学附属肿瘤医院胸外科 [2]830011乌鲁木齐,新疆肿瘤防治研究所

出　　处：《国际呼吸杂志》2013年第16期1231-1235,F0003,共6页International Journal of Respiration

基　　金：新疆医科大学创新基金（XJC201156）

摘　　要：目的 探讨microRNA-10b（miRNA-10b）对人低转移肺癌细胞95-C的增殖、细胞周期、凋亡以及侵袭及迁移能力的影响.方法 用脂质体法将miRNA-10b真核表达质粒瞬时转染入95-C,实验设置空白对照组、空载体转染组和miRNA-10b质粒转染组,转染后荧光显微镜下观察转染效率,实时定量RT-PCR检测各组细胞miRNA-10b的表达,细胞增殖实验检测各组细胞的增殖情况,流式细胞仪检测各组细胞周期及细胞凋亡率,Transwell实验检测各组细胞侵袭能力,细胞划痕实验检测各组细胞迁移能力.结果 转染后miRNA-10b质粒转染组细胞增殖速度明显增快,细胞凋亡率降低,细胞侵袭及迁移能力增强,差异有统计学意义（P＜0.05）.结论 miRNA-10b可以促进95-C细胞的增殖,降低细胞凋亡,增加95-C细胞的侵袭及迁移能力.Objective To investigate the effects of miRNA-10b on proliferation, cell cycle, apoptosis, invasion,and migration of low metastasis of lung cancer cell line （95-C）. Methods Under the induction of Lipofectamine TM 2000, the recombinant of miRNA-10b was transfected into 95-C. The experiment set up three groups： blank control group, empty vector transfected group, and miRNA-10b expression plasmid transfected group. Transfection effciency was observed by fluorescence microscope. miRNA-10b expression level was detected by real-time PCR after transfection. The cell proliferation was detected by cell proliferation assay. The cell cycle and apoptosis were detected by flow cytometry. The invasive ability of cell was detected by Transwell experiment. The migration ability of cell was detected by the wound healing assay in each group. Results Compared with the blank control group and the empty vector transfected group, the cell proliferation rate was obviously increased, the cell apoptosis rate decreased,the cell invasion and migration ability enhanced after transfection （ P ~ 0.05）. Conclusions miRNA-10b can promote the cell proliferation, reduce the apoptosis, and increase the cell invasion and migration ability of 95-C.

关 键 词：MicroRNA-10b 95-C 细胞增殖 细胞周期 细胞凋亡 侵袭能力 迁移能力 

分 类 号：R734.2[医药卫生—肿瘤]