丙泊酚对抑郁大鼠电休克治疗后海马神经元nNOS活性及CAPON表达的影响  

Effects of propofol on nNOS activity and CAPON expression in hippocampal neurons after electroconvulsive therapy in mentally depressed rats

作　　者：律峰[1] 闵苏[1] 沈一维[1] 黎平[1] 李炜[1] 罗洁[1] 李晓[1] 魏珂[1] 陈婧[1] 

机构地区：[1]重庆医科大学附属第一医院麻醉科,400016

出　　处：《中华麻醉学杂志》2013年第7期819-822,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972831,81271501）;重庆市教育委员会科学技术研究项目（KJ090309）;重庆市医学重点学科[渝卫科教（2007）2号];卫生部国家临床重点学科[财社（2011）170号]

摘　　要：目的 评价丙泊酚对抑郁大鼠电休克治疗（ECT）后海马神经元型一氧化氮合酶（nNOS）活性及其羧基末端PDZ结构的接头蛋白（CAPON）表达的影响.方法 清洁级成年雄性SD大鼠,体重200 ～ 250 g,2～3月龄,采用慢性轻度不可预见性应激法建立抑郁模型.选建模成功的大鼠40只,采用随机数字表法,将其分为4组（n=10）：抑郁组（D组）腹腔注射生理盐水8 ml/kg;单纯丙泊酚组（DP组）腹腔注射丙泊酚80 mg/kg；单纯ECT组（DE组）腹腔注射生理盐水8 ml/kg后实施ECT;丙泊酚＋ECT组（DPE组）腹腔注射丙泊酚80mg/kg待翻正反射消失后实施ECT.以上处理1次/d,连续7d.于建模前、建模后、治疗结束后采用糖水偏好实验评价大鼠抑郁状态,采用Morris水迷宫实验测定学习记忆能力.水迷宫实验结束后采用免疫组化法检测海马神经元nNOS及CAPON的表达.结果 与D组比较,DE组、DPE组糖水偏好比升高,DE组逃避潜伏期延长,空间探索时间缩短,DP组海马DG区、CA1区和CA3区神经元nNOS和CAPON表达下调,DE组海马DG区、CA1区和CA3区神经元nNOS表达上调、CAPON表达下调（P＜0.05）；与DE组比较,DPE组逃避潜伏期缩短,空间探索时间延长,海马DG区、CA1区和CA3区神经元nNOS表达下调、CAPON表达上调（P＜0.05）.结论 丙泊酚减轻抑郁大鼠电休克治疗后认知功能损害的机制可能与其抑制海马神经元nNOS活性和上调CAPON表达有关.Objective To evaluate the effects of propofol on neuronal nitric oxide synthase （nNOS） activity and carboxy-terminal PDZ ligand of nNOS （CAPON） expression in hippocampal neurons after electroconvulsive therapy （ECT） in mentally depressed rats.Methods Healthy adult male Sprague-Dawley rats,aged 2-3 months,weighing 200-250 g,were used in this study.Mentally depression was induced by exposing the rats to chronic unpredictable mild stress （CUMS）.Forty mentally depressed rats were randomly divided into 4 groups （n =10 each）：mental depression group （group D）,propofol group （group DP）,ECT group （group DE）,and propofol ＋ECT group （DPE group）.Groups D and DE received intraperitoneal normal saline 8 ml/kg,and in addition group DE received ECT once a day for 7 consecutive days.Groups DP and DPE received intraperitoneal propofol 80 mg/kg,and in addition after the righting reflex disappeared,group DPE received ECT once a day for 7 consecutive days.All rats underwent sucrose preference test and Morris water maze test before CUMS,after CUMS,and after the end of treatment.The rats were sacrificed after completion of Morris water maze test and hippocampi were removed for determination of nNOS and CAPON expression in hippocampal neurons.Results Compared with group D,the sucrose preference percentage was significantly increased in DE and DPE groups,the escape latency was prolonged and space exploration time was shortened,the expression of nNOS in hippocampal DG,CA1 and CA3 regions was up-regulated and the expression of CAPON in hippocampal DG,CA1 and CA3 regions was down-regulated in group DE,and the expression of nNOS and CAPON in hippocampal DG,CA1 and CA3 regions was downregulated in group DP （P ＜ 0.05）.Compared with group DE,the escape latency was significantly shortened and space exploration time was prolonged,the expression of nNOS in hippocampal DG,CA1 and CA3 regions was down-regulated and the expression of CAPON in hippocampal DG,CA1 and CA3 regions was up-regulated in group DP

关 键 词：二异丙酚 电惊厥疗法 抑郁 一氧化氮合酶Ⅰ型 海马 

分 类 号：R614[医药卫生—麻醉学]