电压门控钠离子通道β3亚基在小鼠神经病理性痛中的作用  

Role of beta3 subunit of voltage-gated sodium channels in neuropathic pain in mice

作　　者：赵璇[1] 谭弘[2] 张璐[2] 陈治宇[2] 王英伟[2] 俞卫锋[1] 

机构地区：[1]第二军医大学附属东方肝胆医院麻醉科,上海市200433 [2]上海交通大学医学院附属新华医院麻醉与重症医学科

出　　处：《中华麻醉学杂志》2013年第7期864-865,共2页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30872443）

摘　　要：目的 评价电压门控钠离子通道β3亚基（Scn3b）在小鼠神经病理性痛中的作用.方法 采用转基因手段,将目的基因Scn3b嵌入pBROAD-mcs载体中,成为pBROAD-rosa-Scn3b质粒,孕育原代小鼠.将原代小鼠和C57/B6小鼠进行配种,得到转基因小鼠.通过DNA、RT-PCR和Western blot实验鉴定过表达成功后,进行后续实验.随机选择Scn3b过表达转基因小鼠（Scn3b组）和同窝阴性对照小鼠（Con组）各10只,2月龄,雌雄不拘,体重25 ～ 30 g,制备神经病理性痛模型.分别于术前、术后3、5、7和14 d时,测定机械痛阈.结果 2组间不同时间点机械痛阈比较差异无统计学意义（P＞0.05）.结论 Scn3b不参与小鼠神经病理性痛的形成和维持.Objective To evaluate the role of the beta3 subunit of the voltage-gated sodium channel （Scn3b） in neuropathic pain in mice.Methods The target gene Scn3b was embedded in the vector pBROAD-mcs and pBROAD3-mcs-Scn3b plasmid was then obtained.The primary mice were bred.The primary mice mated with C57/B6 mice and the transgenic mice were then generated.DNA,RT-PCR and Western blot experiments were performed to confirm the mice in which Scn3b was over-expressed.The mice with Scn3b over-expression multiplied rapidly to carry out the follow-up experiment.Ten transgenic mice （Scn3b group） and 10 control mice in the same litter （Con group） of both sexes,aged 2 months,weighing 25-30 g,were randomly chosen to establish the model of neuropathic pain.The mechanical pain threshold was measured before operation and on 3,5,7 and 14 days after operation.Results There was no significant difference in the mechanical pain threshold at each time point between the two groups （P ＞ 0.05）.Conclusion Scn3b is not involved in the development and maintenance of neuropathic pain in mice.

关 键 词：钠通道 神经痛 

分 类 号：R741.041[医药卫生—神经病学与精神病学]