子宫内膜癌及不同子宫内膜病变组织中p57^(kip2)、ER、PR的表达及意义  

Expressions and significances of p57^(kip2),estrogen receptor,progesterone receptor in endometrial carcinoma and endometrial different lesions

作　　者：罗祖强[1] 周正平[2] 格桑志玛[1] 肖庆邦[1] 

机构地区：[1]遵义医学院病理学教研室,遵义563099 [2]遵义医学院医学与生物学研究中心电镜室,遵义563099

出　　处：《临床与实验病理学杂志》2013年第9期982-985,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：贵州省教育厅自然科学研究基金(黔教科2010047)

摘　　要：目的探讨p57kip2、ER、PR在子宫内膜癌发生、发展中的作用。方法采用免疫组化EliVision法检测100例子宫内膜样腺癌(endometrioid adenocarcinoma,EA)、20例子宫内膜上皮内瘤变(endometrial intraepithelial neoplasia,EIN)、20例增生性病变、20例增生期和20例分泌期组织中p57kip2、ER、PR蛋白的表达。结果 p57kip2蛋白在子宫内膜分泌期中表达最高,在增生性病变中表达最低,在EA中表达低于在EIN、分泌期中的表达,且高于在增生性病变、增生期中的表达;p57kip2在分泌期中的表达与EA、增生性病变、增生期中的表达相比,差异有统计学意义(P<0.01)。ER蛋白在增生性病变中的表达最高,在EA中的表达最低。ER蛋白在EA中的表达与在增生性病变、增生期中的表达相比,差异有显著性(P<0.01)。PR蛋白在增生性病变、增生期中的表达最高,在EA中的表达最低,但在各组病变中的表达相比,差异均无显著性(P>0.05)。在EA中,p57kip2、ER、PR蛋白的表达均与组织学分级有关(P<0.05)。p57kip2与PR、ER与PR的表达均呈正相关(P<0.01)。结论p57kip2、ER、PR蛋白的异常表达可能是子宫内膜组织恶性转变的重要生物学标志;孕激素可促进p57kip2蛋白的表达,协同负调控细胞周期、阻滞EA的发生、发展;雌激素可下调p57kip2蛋白的表达,推进细胞周期进程、促进EA的发生、发展。Purpose To explore the effects of p57kip2, estrogen receptor （ER） and progesterone receptor（PR） on the carcinogenesis and progression of endometrial carcinoma. Methods The expression of p57kiv2, ER and PR proteins in 100 cases of endometrioid ade- nocarcinoma （EA） , 20 cases of intraepithelial neoplasia （EIN） , 20 cases of hyperplasia lesion, 20 cases of proliferative phase and 20 cases of secretory phase were detected by immunohistochemical EliVision methods, respectively. Results The highest expression of p57kip2 protein was in endometrial secretory phase and the lowest expression of p57kip2 protein was in hyperplasia lesions. The expression of p57kip2 protein in EA was lower than that in EIN and secretory phase, but higher than that in hyperplasia lesions and proliferative phase, but only the expression of p57klp2 protein in secretory phase was higher than that in EA, hyperplasia lesions and proliferative phase （P 〈 0. 01 ）. The highest expression of ER protein was in hyperplasia lesions and the lowest expression of ER protein was in EA. The expression of ER protein showed significant difference between hyperplasia lesions and EA, and between proliferative phase and EA （ P 〈 0. O1 ）. The expression of PR protein in hyperplasia lesions and proliferative phase was the highest. The expression of PR protein in EA was the lowest. But there was no significant differences among the expression of PR protein in all lesions （P 〉 0. 05 ）. In EA, there was a correlation between the respective expression of p57kip2 proteins, ER proteins and PR proteins with histological grade （P 〈 0. 05 ）. There was positive correlation between expression of p57kip2 and PR, and between expression of ER and PR （ P 〈 0. O1 ）. Con- clusion The abnormal expression of p57kip2 , ER and PR protein might be important biological markers for malignant transformation in endometrial tissue. Progestogen may promote the expression of p57kip2 protein and they may have a synergistical role in negative regula-

关 键 词：子宫肿瘤 子宫内膜样腺癌 P57KIP2 ER PR 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]