甲状腺乳头状癌中HIF-1α、VEGF、COX-2及VEGF-C表达与侵袭转移的关系  

Relationship between the expression of HIF-1α,VEGF ,COX-2 and VEGF-C and invasion, metastasis of human papillary thyroid carcinoma

作　　者：杜日昌[1,2] 李宇芳[1,3] 邱卫东[1,4] 陈智慧[1,2] 谭丽珊[1,2] 陈玉英[1,2] 李海南[1,2] 郭慧[1,2] 张振斌[1,2] 

机构地区：[1]广东医学院附属韶关医院 [2]韶关市第一人民医院病理科,韶关512000 [3]韶关市第一人民医院骨科,韶关512000 [4]韶关市第一人民医院麻醉科,韶关512000

出　　处：《临床与实验病理学杂志》2013年第9期991-995,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：韶关市科技计划项目[韶科(卫)2010-25]

摘　　要：目的探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中缺氧诱导因子1α(hypoxia inducible factor-1α,HIF-1α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、COX-2和VEGF-C的表达及与血管及淋巴管生成的关系。方法采用免疫组化EnVision法检测PTC中HIF-1α、VEGF、COX-2与VEGF-C的表达,并用CD34与D2-40双重免疫组化技术标记检测微血管密度(micro-vascular density,MVD)及微淋巴管密度(micro-lymphatic vessel density,MLVD),结合临床病理学特征和随访资料进行分析。结果 PTC中HIF-1α、VEGF、COX-2及VEGF-C的阳性率分别为79.5%、72.6%、82.2%和89.0%,均高于非毒性甲状腺肿(P<0.05)。PTC中有淋巴结转移者HIF1α、VEGF、COX-2和VEGF-C表达均高于无淋巴结转移者(P<0.05),HIF-1α、VEGF、COX-2与VEGF-C的表达存在相关性(P<0.05)。淋巴结转移组的MVD及MLVD高于无淋巴结转移组(P<0.05)。结论 PTC中HIF-1α、VEGF、COX-2和VEGF-C的高表达可能促进肿瘤的侵袭和转移,且VEGF、VEGF-C的表达可能由HIF-1α和COX-2协同调节,共同参与淋巴结转移过程。Purpose To investigate the relationship between the expression of HIF-la, VEGF, COX-2 and VEGF-C and angiogenesis and lymphangiogenesis in human papillary thyroid carcinoma （PTC）. Methods HIF-la, VEGF, COX-2 and VEGF-C expression were detected by EnVision immunohistochemical method in 74 patients of PTC and 30 of nontoxic goiter. Double immunohistochemical staining technique was used to observe the MLVD and MVD. Results Positive rates of HIF-la （79. 5% ）, VEGF （72. 6% ）, COX- 2 （82. 2% ） and VEGF-C （89.0%） in PTC were higher than those in nontoxic goiter （P 〈 0. 05）. In carcinoma patients with lymph node metastasis, the higher expressions of HIF-1α, VEGF, COX-2 and VEGF-C were observed as compared with those without metas- tasis （ P 〈 0. 05 ） , respectively. There were positive correlations between the expression of HIF-1 oL, VEGF, COX-2 and VEGF-C （ P 〈 0. 05 ）. The MVD and MLVD of lymph node metastasis group was significantly higher than that of lymph node non-metastasis group （ P 〈 0. 05）. Conclusion In PTC, the HIF-lc~, VEGF, COX-2 and VEGF-C may play an important role in papillary thyroid cancer pro- gression, VEGF and VEGF-C expression may be regulated by HIF-lα and COX-2, involving in the process of cancer cell spreading to lymph nodes.

关 键 词：甲状腺肿瘤 缺氧诱导因子lα 血管内皮生长因子 环氧化酶-2 血管内皮生长因子C 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]