NIRS法快速测定复方阿胶浆中总黄酮、总皂苷和可溶性固形物  

Rapid determination of total flavonoids, total saponins, and soluble solid content in Fufang Ejiao Syrup by NIRS

作　　者：韩海帆[1] 张路 张淹 李文龙[1] 瞿海斌[1] 

机构地区：[1]浙江大学药物信息学研究所,浙江杭州310058 [2]国家胶类中药工程技术研究中心,山东聊城252201

出　　处：《中草药》2013年第17期2397-2403,共7页Chinese Traditional and Herbal Drugs

基　　金：国家“重大新药创制”科技重大专项(2011ZX09201-201-10);浙江省公益技术研究项目(2012C21102)

摘　　要：目的利用近红外光谱(NIRS)快速测定复方阿胶浆中总黄酮、总皂苷和可溶性固形物。方法采用比色法测定复方阿胶浆中总黄酮和总皂苷的量,采用水分测定仪测定可溶性固形物的量,作为参考值。运用偏最小二乘回归(PLSR)法建立NIRS与各项指标的量参考值之间的多元校正模型。结果校正模型的预测相关系数(R2)和预测误差均方根(RMSEP)分别为总黄酮0.979 8,69.6μg/mL;总皂苷0.966 0,17.6μg/mL;可溶性固形物0.997 8,0.394%。结论本法简捷快速,结果准确,可用于复方阿胶浆成品的快速检测和质量控制。Objective To develop an expeditious method for the simultaneous determination of main active compounds in Fufang Ejiao Syrup （FES, compound Ejiao oral liquid） by near infrared spectroscopy （NIRS） in transflective mode. Methods The contents of total flavonoids and total saponins were determined by colorimetric assays, while the soluble solid content was determined using a moisture analyzer as reference value. Partial least squares regression （PLSR） was employed for the calibration of regression models between NIRS and the indexes. Results The correlation coefficients （R2） of the constructed models for total flavonoids, total saponins, and soluble solid content were 0.979 8, 0.966 0, and 0.997 8, respectively, and the root mean square errors of prediction （RMSEP） were 69.6, 17.6 μg/mL, and 0.394%, respectively. Conclusion The proposed method is fast and accurate, and facilitating the rapid analysis of main active components in FES.

关 键 词：近红外光谱 总黄酮 总皂苷 可溶性固形物 复方阿胶浆 

分 类 号：R286.02[医药卫生—中药学]