ALOX5AP基因多态性与动脉粥样硬化性脑梗死相关性  

ASSOCIATION OF ALOX5AP POLYMORPHISMS WITH ATHEROSCLEROTIC CEREBRAL INFARCTION

作　　者：姚倩倩[1] 周畅[1] 刘丽君[1] 袁荣荣[1] 张晨[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003

出　　处：《青岛大学医学院学报》2013年第6期471-475,共5页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金资助项目(ZR2010HM061)

摘　　要：目的探讨5-脂氧合酶激活蛋白（ALOX5AP）基因多态性与动脉粥样硬化性脑梗死（ACI）的相关性。方法应用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）分析法，检测412例ACI病人和472例性别、年龄与ACI病人相匹配的健康人群ALOX5AP基因（SGl3S114、SGl3S89、SGl3S32）多态性。结果病例组和对照组相比，SGl3S114位点等位基因T和基因型TT／TA分布差异具有统计学意义（x^2=21．63、10．99，P〈O．01）。应用多因素Logistic回归方法排除传统危险因素的影响后，携带SGl3S114TT／TA基因型和SG13S32CC／CA基因型的人群ACI发病风险分别是携带AA基因型人群的2．35倍（OR=2．35，95％CI=1．46～3．78，P〈0．01）和1．43倍（OR=1．43，95％CI=1．02～2．01，P〈O．05）。经传统危险因素糖尿病分层后，SGl3S32CC／CA基因型能够明显增加糖尿病人群患ACI的风险（OR=3．27，95％CI=1．55～6．88，P〈o．01）。单倍型TGC作为一种危险单倍型（OR=1．46，95％CI=1．17～1．82，P〈0．01）可以增加ACI的发病风险。结论ALOX5AP基因多态性与ACI的发生密切相关，且该基因可明显增加糖尿病人群ACI的发病风险。Objective To investigate the association of 5-1ipoxygenase activating protein （ALOX5AP） gene polymor- phisms with atherosclerotic cerebral infarction （ACI）. Methods By using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis, three polymorphisms of the activating 5-1ipoxygenase （ALOXSAP） gene （SG13Sl14, SG13S89,SG13S32） were analyzed in 412 patients with ACI and 472 age- and sex-matched healthy controls. Results Significant differences at allele T and genotype TT/TA distributions of SG13S114 were observed between the ACI and the control （x^2 ： 21.63, 10.99 ;P〈0.01）. Multivariate logistic regression analysis revealed that SG13Sl14 genotype TT/TA and SG13S32 genotype CC/CA had a 2.35-fold （OR ：2.35,95 %CI= 1.46--3.78,P〈0.01） and a 1.43-fold （OR ： 1.43,95%CI： 1.02--2.01,P〈0.05） increased risk for ACI patients compared with AA genotype, respectively. Stratification based on the status of history of diabetes revealed that SG13S32 genotype CC/CA increased the risk of ACI in patients with diabetes （OR ： 3.27,95 % CI ： 1.55 -- 6.88, P （0.01）. The haplotype analysis showed that the haplotype TGC （OR = 1.46,95 % CI = 1.17 - 1.82, P 〈 0.01） was associated with an in- creased risk for ACI. Conclusion Genetic polymorphism of ALOXSAP was closely associated with ACI and it can obviously in- crease the risk of ACI in patients with diabetes.

关 键 词：花生四烯酸盐5-脂氧合酶 动脉粥样硬化 脑梗死 多态性 单核苷酸 白三烯类 

分 类 号：R743.33[医药卫生—神经病学与精神病学]