原发性肝癌患者HBVDNA水平和基因分型及HBV-M检测的相关性分析  

Analysis of relationship between hepatitis B virus genotyping and HBV DNA level and serologic markers in primary hepatic cancer

作　　者：张正国[1] 贺庆伟[1] 张晋军[1] 

机构地区：[1]河南省中医院检验科,河南郑州450002

出　　处：《中国卫生检验杂志》2013年第11期2501-2503,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨原发性肝癌(PHC)患者HBV DNA水平与HBV基因分型及乙型肝炎病毒血清标志物(HBV-M)的相关性。方法收集228例原发性肝癌患者(PHC组),同时选取145例慢性乙型肝炎患者(CHB组)作为对照,应用聚合酶链反应(PCR)和酶联免疫吸附试验(ELISA)对两组患者的HBV DNA载量及HBV-M进行检测,HBV DNA阳性者采用基因测序技术对PCR扩增后产物进行基因分型。结果 HBV DNA阳性率PHC组为71.05%(162/228),CHB组为74.48%(108/145),差异无统计学意义。两组HBV基因分型最常见为B型和C型,未检出其他基因型。PHC组HBV基因型以C型为主占57.89%(132/228),其次为B型占11.84%(27/228);CHB组HBV基因型C型占58.62%(85/145),B型占13.79%(20/145)。结论本地区PHC组患者HBV感染率较高,且与HBV感染的病毒复制密切相关,HBV-M以小三阳最为常见,HBV基因型以C型为主,HBV DNA水平稍低于CHB组。HBV-M模式为小三阳的慢性乙型肝炎患者是肝癌的高危人群,更要警惕PHC的发生,应重视该人群的定期体检和复查。Objective To investigate the relationship between serum hepatitis B viral HBV DNA and genotype and HBV mark- ers in patients with primary hepatic cancer. Methods Two hundred and twenty - eight patients with primary hepatic cancer and 145 patients with chronic hepatitis B were enrolled ,and serum markers of hepatitis B virus were detected by ELISA and HBV DNA by fluorescent quantitative polymerase chain reaction. Gene sequencing of PCR products for genotyping were used in HBV DNA positive patients. Results The HBV DNA of hepatitis B virus in 228 patients （71.05%） with primary hepatic cancer were positive, while serum HBV DNA positive rate was 74.48% （108/145）in 145 patients with chronic hepatitis B;there was no difference between the two. Type B and type C were the most common HBV genotype, other genotypes were not detected＇of 228 primary hepatic cancer patients. In PHC group, 57.89% were genotype C, 13.79% were genotype B and 58.62% were genotype C, 13.79% were genotype B in patients with chronic hepatitis B. Conclusion The local infection rate of hepatitis B virus was high in patients with primary hepatic cancer, and it closely related to the replication of the virus. HBV - M positive and genotype C were most common, and the level of HBV DNA was slightly lower in patients with chronic hepatitis B. The chro- nic hepatitis B patients with HBV - M positive were at higher risk of hepatocarcinoma, so more attention should be paid to the crowdg regular physical examination and review.

关 键 词：原发性肝癌 基因分型 HBV—DNA 血清标志物 慢性乙型肝炎 

分 类 号：R735.7[医药卫生—肿瘤]