氟病黄牛自由基损伤及硒铜镁保护作用机理的研究  

Study on the Free Radical induced Damage in Cattle with Endemic Fluorosis and the Protective Mechanism of Selenium,Copper,and Magnesium

作　　者：韩博[1] 尹文利 史言[2] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]东北农业大学动物医学院

出　　处：《中国农业科学》2000年第6期80-87,共8页Scientia Agricultura Sinica

摘　　要：:32头高氟黄牛随机分为 4组 ,每组 8头 ,其中 组为高氟对照 ; 组为高氟并每日添加 0 .2 5mg/kg硒 ; 组为高氟并每日添加 1 5mg/kg铜 ; 组为高氟并每日添加 0 .2 5mg/kg硒+1 5mg/kg铜 +1 mg/kg镁。另设正常对照组奶、黄牛各 8头 ,连续饲喂 83d,分别于试验的第0、30、83天采样 ,检测结果表明 :高氟黄牛 GSH- px、CAT和 SOD活性显著低于正常对照奶、黄牛 ,MDA和自由基含量显著高于正常对照奶、黄牛。高氟加硒和加硒铜镁黄牛第 30天和第 83天的 GSH- px、CAT和 SOD活性分别比同组第 0天和相同时间的高氟对照黄牛升高 ,MDA和自由基含量分别比同组第 0天和相同时间的高氟对照黄牛显著降低。添加硒铜镁使高氟黄牛抗氧化酶类活性第 30天时明显升高 ,脂质过氧化物和自由基含量下降。加硒使高氟黄牛抗氧化酶类活性到第 83天升高 ,其抗氧化作用不及硒铜镁联合添加组。表明适宜剂量的硒铜镁联合作用 ,使高氟低硒低铜黄牛体氟排泄增加 ,脂质过氧化产物和自由基生成减少 。The changes of anti oxidative enzymes, lipid peroxidation and free radicals were observed in cattle with endemic fluorosis under high fluoride, low selenium and low copper environment. Thirty two cattle were selected from high fluoride area and randomly divided into four groups with eight cattle each as follows: Ⅰ high fluoride control group (HFC); Ⅱ supplemented with 0.25mg/kg selenium (HFSe); Ⅲsupplemented with 15mg/kg copper(HFCu);and Ⅳ supplemented with 0.25 mg/kg selenium+15mg/kg copper+1mg/kg magnesium (HFSeCuMg) per day for 83 d, respectively. Eight cattle and eight dairy cows were selected from non high fluoride area as normal control groups.The samples were collected and measured on 0d, 30d,and 83d, respectively. The results showed that the contents of relative free radicals and MDA in the high fluoride group were significantly higher, and the whole blood GSH Px, CAT, erythrocyte SOD activities were lower than that of the control groups. So, free radicals metabolic imbalance and antioxidation disorder were the main factors in endemic fluorosis. GSH Px, CAT and SOD activities in HFSe group and HFSeCuMg group at 30d and 83d were markedly higher than that of the same group at 0d and the same time HFC. There was a corresponding reduction in the contents of relative free radicals and MDA. Supplementation of selenium, copper and magnesium increased high fluoride bovine anti oxidative enzemyes at 30 days, and decreased MDA and free radicals contents. However, the activities of anti oxidative enzymes in group supplementated with selenium did not increase until the 83 days, so the anti oxidative function of the group supplemented with Se,Cu,and Mg was better than that of the groupsupplemented with Se. The results showed that the high fluoride affected cattle supplemented with proper selenium, copper,and magnesium increased their fluoride excretion and anti oxidative function, and decreased lipid peroxidation and free radicals contents.

关 键 词：黄牛 氟中毒 GSH-px SOD CAT MDA 自由基 硒 铜 镁 

分 类 号：S856.9[农业科学—临床兽医学]