肺腺癌中LDH-V、AKT1及Glut1的表达及其与18氟-2-脱氧葡萄糖摄取的相关性研究  

Expression of LDH-V,AKT1,Glut1and Their Relationships with the Uptake of 18F-FDG in Lung Adenocarci noma

作　　者：李倩[1] 黄钢[2] 刘建军[2] 孙晓光[1] 

机构地区：[1]上海交通大学医学院附属仁济医院核医学科,上海200127 [2]上海交通大学医学院,上海200025

出　　处：《中国临床医学》2013年第4期434-436,442,共4页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金资助项目(编号:81071180;30830038;30970842)

摘　　要：目的:探讨肺腺癌中乳酸脱氢酶-V(lactate dehydrogenase V,LDH-V)、丝氨酸/苏氨酸蛋白激酶1(serine-threonine kinase 1,AKT1)及葡萄糖转运蛋白1(glucose transporter 1,Glut1)的表达及它们与18氟-2-脱氧葡萄糖(18F-FDG)摄取的关系。方法:54名肺腺癌患者,术前1周进行PET-CT显像。采用免疫组织化学SP法对LDH-V、AKT1、Glut1的表达进行半定量分析,并与术前PET-CT所得的18F-FDG最大标准化摄取值(maximal standardized uptake value,SUVmax)进行相关性分析。结果:在肺腺癌中,LDH-V、AKT1及Glut1表达的阳性率为88.89%、88.89%、68.52%,LDH-V表达与AKT1及Glut1表达呈正相关(r分别为0.381和0.270,P<0.01和0.05);SUVmax与肿瘤最大直径(maximal diameter,Dmax)呈正相关(r=0.524,P<0.01),Dmax>2 cm患者(n=30)的SUVmax与Glut1表达呈正相关(r=0.407,P<0.05);SUVmax与病理分级、LDH-V及AKT1表达无关。结论:LDH-V、AKT1及Glut1在肺腺癌中广泛表达且LDH-V表达与AKT1及Glut1表达密切相关,Glut1在肺腺癌18F-FDG摄取中发挥重要作用。Objective：To investigate the expression of lactate dehydrogenase V（LDH-V）,serine-threonine kinase 1（AKT1）,glucose transporter 1（Glut1）and their relationships with the uptake of 18F-FDG in lung adenocarcinoma.Methods：A total of 54 patients with lung adenocarcinoma were involved.All patients were examined by PET-CT one week before the operation.The expression levels of LDH-V,AKT1 and Glut1 were detected by SP immunohistochemistry method and assessed by semiquantitative analysis.Correlation analysis was carried out to evaluate correlations of their expression with the uptake of 18F-FDG.Results：Positive expression of LDH-V,AKT1 and Glut1 were found in 88.89 %,88.89 % and 68.52 % of the 54 patients,respectively.Significantly positive correlations were found between the expression of LDH-V and AKT1（r=0.381,P〈0.01）,as well as between LDH-V and Glut1（r=0.270,P〈0.05）.Maximal standardized uptake value（SUVmax）was positively correlated with maximal diameter（Dmax）,r=0.524,P〈0.01.SUVmax was positively correlated with the expression of Glut1 in patients whose Dmax2 cm（n=30）,r=0.407,P〈0.05.SUVmax had no correlation with pathological grading and the expression of AKT1 or Glut1.Conclusions：LDH-V,AKT1 and Glut1 are widely and obviously expressed in lung adenocarcinoma.The expression of LDH-V was positively correlated with AKT1 and Glut1.Glut1 plays an important role in the uptake of 18FFDG.

关 键 词：肺腺癌 乳酸脱氢酶-V 丝氨酸 苏氨酸蛋白激酶1 葡萄糖转运蛋白1 最大标准化摄取值 

分 类 号：R734.2[医药卫生—肿瘤]