茶多酚对苯并(a)芘诱导的人单核细胞热休克蛋白70和生长分化因子-15表达的影响  

Effects of tea polyphenols on HSP70and GDF-15expression of monocyte induced by benzo(a)pyrene in vitro

作　　者：刘红云[1] 包永芬[1] 单士刚[1] 

机构地区：[1]湖北科技学院基础医学院,437100

出　　处：《山西医药杂志（上半月）》2013年第10期1093-1095,共3页Shanxi Medical Journal

基　　金：湖北省教育厅科学技术研究项目(Q20082806;Q20132804)

摘　　要：目的研究茶多酚对苯并(a)芘诱导的人单核细胞热休克蛋白(HSP70)和生长分化因子-15(GDF-15)表达的影响。方法健康人单核细胞,按完全随机实验设计分成4个组:①空白对照组;②溶剂对照组[(1‰二甲基亚砜(DMSO),3‰S9];③苯并(a)芘染毒组,以不同浓度苯并(a)芘(0.1、0.5、1、5、10μmol/L)染毒24h;④茶多酚预处理组,以50μg/mL的茶多酚预处理6h后,再以不同浓度苯并(a)芘(0.1、0.5、1、5、10μmol/L)染毒24h,分别应用蛋白印迹法和酶联免疫吸附试验(ELISA)法研究茶多酚对苯并(a)芘诱导的单核细胞HSP70和GDF-15表达情况的影响。结果苯并(a)芘抑制了单核细胞HSP70表达,同时促进GDF-15的分泌;茶多酚预处理组单核细胞HSP70表达与对照组相比差异无统计学意义(P<0.05)。茶多酚预处理组GDF-15分泌没有显著性变化。结论茶多酚可以提高HSP70表达,从而通过内源性的保护机制抵制苯并(a)芘对单核细胞造成的损伤,同时细胞本身通过提高GDF-15表达,降低苯并(a)芘对单核细胞造成的损伤。Objective To investigate the effects of tea polyphenols on HSP70 and GDF-15 expression in monocyte induced by benzo（a）pyrene[B（a）P]. Methods Human monocytes were randomized into 4 groups：（ Ⅰ ） blank control group （ Ⅱ ） solvent control group; （Ⅲ ） B（a） P group exposed to B（a） P at the concentration of 0.1, 0.5, 1, 5 and 10 μmol/L, in culture medium for 24 hours： （Ⅳ） cells were pretreated with TP for 6 hours before treated with B（a）P as above in TP-pretreated groups, Western blot was used to measure the expression of HSP70 and the supernatants were collected and analyzed by ELISA to quantify the release of GDF-15 from the cells. Results B（a）P inhibited the expression of HSP70 in TP-pretreated groups and GDF-15 secretion increased with B （a）P increasing. Compared with the control group, there was statistical significance （ P d0.05） in a dose-effect relationship. There was not significant difference in HSP70 expression levels among TP-pretreated groups and control group. Conclusion It is suggested that TP could improve HSP70 expression, which activate the endoge- nous protective mechanism against B（a）P damage to the monocytes, meanwhile, by increasing GDF-15 expression to reduce B（a）P damage for cells.

关 键 词：单核细胞 热休克蛋白质70 生长分化因子-15 苯并芘 

分 类 号：R363[医药卫生—病理学]