不同加工方法对北沙参多糖免疫调节功能的实验研究  

Assessment of preliminary processing and its experimental study on the immunoregulation function of Radix Glehniae polysaccharide

作　　者：荣立新[1] 鲁爽[1] 刘咏梅[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国中医基础医学杂志》2013年第9期846-847,861,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的:通过比较不同加工方法制得的北沙参中粗多糖(Polysaccharide of Radix Glehnia,GLP)对阴虚小鼠的免疫调节作用,探讨北沙参加工过程中去皮和不去皮的意义。方法:采用去皮和不去皮2种方法加工北沙参,分别提取去皮北沙参中的粗多糖(GLP1)和未去皮北沙参中的粗多糖(GLP2);制备阴虚小鼠模型,观察小鼠体质量变化,检测脾脏NK细胞杀伤活力、T淋巴细胞转化功能和小鼠血清抗绵羊红细胞抗体IgM含量。结果:GLP1和GLP2均可使阴虚小鼠体质量显著增加(P<0.05或P<0.01);显著增强甲亢型阴虚小鼠脾脏NK细胞杀伤率(P<0.05)和T淋巴细胞转化功能(P<0.01,P<0.05),提高血清IgM含量;且两种北沙参粗多糖(GLP1、GLP2)各相同剂量组之间的作用差异无统计学意义(P>0.05)。结论:两种北沙参粗多糖GLP1和GLP2在增强机体免疫功能方面差异无统计学意义,说明北沙参可以不去皮应用,为改进北沙参的传统加工方法提供了实验依据。Objective： To discuss the significance of preliminary processing in peeling and not peeling Radix Glehniae by camparison with immuno-regulation function in Yin-deficiency mice of different processing methods prepared Radix Glehniae polysaccharide. Methods：Peeling and not peeling Radix Glehniae processing were applied,the polysaccharide of peeling Radix Glehniae （GLP1） and the polysaccharide of not peeling Radix Glehniae （GLP2）were extracted separately;the Hyperthyroidism Yin-deficiency mice were prepared , and the effects of GLP1 and GLP2 on changes of weight, killing activity of NK cells,the T lymphocyte transformation function in spleens and the content of serum anti sheep red blood cell antibodies IgM in Yin deficiency mice were separately determined. Results：GLP1 and GLP2 could obviously increased the weights（P 〈 0.05 or P 〈 0. 01 ） , and also significantly enhanced the killing activity of NK cells （ P 〈 0. 05 ） and the T lymphocyte transformation function, raised the quantity of serum antibody IgM （ P 〈 0.05 or P 〈 0.01 ） ; there was no significant difference between the same dose group of GLP1 and GLP2 （ P 〉 0. 05 ）. Conclusion： GLP1 and GLP2 had no significant differences on the immune function , this shows that Radix Glehniae could be not peeled application. This study provided an experimental basis for the improvement of the traditional processing method of Radix Glehniae.

关 键 词：北沙参 产地加工 粗多糖 免疫调节 

分 类 号：R285.5[医药卫生—中药学]