三聚氰胺分子印迹传感器研制及其应用  

Melamine Molecular Imprinting Electrochemical Sensor: Research and Development

作　　者：栾崇林[1] 麦家超[1] 李俊武[1] 蒋晓华[1] 代建国[1] 金刚[1] 

机构地区：[1]深圳职业技术学院应用化学与生物技术学院,广东深圳518055

出　　处：《深圳职业技术学院学报》2013年第5期44-48,共5页Journal of Shenzhen Polytechnic

基　　金：深圳市科技计划资助项目(SY200806270046A)

摘　　要：以邻氨基酚（OAP）为单体，三聚氰胺为印迹分子，在玻碳电极上电化学聚合制备了三聚氰胺分子印迹敏感膜．分子印迹传感器敏感膜洗脱前和洗脱后在电化学特性方面有明显的不同．以K3[Fe（CN）6]为电化学探针，利用差分脉冲法（DPV）研究了传感器的响应性能，在三聚氰胺浓度0．4-1．0μg／kg和2．0-10μg／kg范围内，传感器峰电流变化（△i）与三聚氰胺浓度C成线性关系，线性方程分别为：△i=281200c-0．1059，R=O．997：Ai=13410c＋0．1735，R=0．999．定量检测下限为0．4μg／kg．该传感器的响应时间为5min，测定相对标准偏差为3．46％（n=71，有较好的选择性，用于实际样品测定，加标回收率为88．3％-110％．Molecularly imprinted films （MIP） were prepared in pH 6.80 PBS buffer solution by cyclic voltammetric electropolymerization method on glassy carbon electrode with o-aminophenol acting as monomer and melamine acting as imprinted molecules. Melamine molecularly imprinted electrochemical sensor was obtained after removing imprinted molecules-melamine from the films. The significant differences in electrochemical signals of the sensors were observed between before and after removal of imprinted molecules. The response characteristic of the MIP sensor was studied by differential pulse voltammetry （DPV） using K3[Fe（CN）6] as probe. The concentrate of melamine in the respective range of 0.4-1.0μg/L and 2.0-10μg/L presented excellent linear relationship to its change in peak currents （△i） and its linear regression equation are Ai =0.2812c-0.1059, R=0.997 and △i =0.01341 c＋0.1735, R=0.999 respectively. The limit of quantification was 0.4μg/kg and the response time was about 5min. The relative standard deviation of the sensor for determination of melamine was 3.46% （n = 7） with good selectivity. It was applied in determination of melamine in milk and milk powder samples, and the spiked recoveries were 88.3% to 110%.

关 键 词：玻碳电极 分子印迹 三聚氰胺 电化学传感器 

分 类 号：TS207[轻工技术与工程—食品科学] O657[轻工技术与工程—食品科学与工程]