白藜芦醇下调NF-κB介导的MMP-9表达抑制人NCI-H446细胞的侵袭迁移  

Resveratrol Suppresses NF- κB- mediated MMP- 9 Activity to Inhibit Human Small Cell Lung Cancer Cells Immigration and Invasion

作　　者：刘玉斌[1] 曹爱国[1] 李之茂[1] 谢静[2] 

机构地区：[1]湘南学院附属医院呼吸内科,湖南郴州423000 [2]郴州市第一人民医院

出　　处：《实用预防医学》2013年第9期1141-1144,共4页Practical Preventive Medicine

摘　　要：目的观察白藜芦醇对肺癌细胞基质金属蛋白酶9(MMP-9)表达的影响,并探讨其可能的分子机制。方法体外培养人小细胞肺癌NCI-H446细胞,用不同浓度的白藜芦醇刺激。小室侵袭实验检测NCI-H446细胞的迁移与侵袭;Western blot检测MMP-9表达以及NF-κB p65亚基核转位;荧光素酶报告基因分析MMP-9和NF-κB活性。结果 50 nM白藜芦醇孵育24 h后,其迁移与侵袭率分别降低了37%和33%;Western blot显示,白藜芦醇能抑制p65核转位;此外,白藜芦醇能以剂量依赖性方式抑制MMP-9的转录活性,NF-κB突变后报告基因活性显著降低。结论白藜芦醇可通过抑制NF-κB介导的MMP表达从而抑制NCI-H446细胞侵袭迁移。Objective To observe the effect of resveratrol on matrix metalloproteinase- 9 （MMP - 9） expression in hu- man small cell lung cancer cells, and then explore its possible molecular mechanism. Methods Human small ceil lung cancer line NCI - H446 was cultured in vitro, and stimulated by different concentrations of resveratrol. The migratory and invasive abilities were assessed by chamber invasion assay. Expression of MMP - 9 and translocation of NF - κ p65 subunit were detected by Western blot. Transcription of MMP - 9 was detected by lueiferase reporter gene assay. Results After the treatment of NCl- H446 cells with resveratrol （50 nM） for 24 hours, the migratory and invasive abilities were significantly decreased （37% and 33 % inhibition, respectively）. Western blot showed resveratrol could inhibit the translocation of p65. Furthermore, resvera- trol inhibited promoter activity of MMP - 9 in a dose- dependent manner, and the promoter activities of NF - κB deletion muta- nts were significantly affected by resveratrol. Conclusions The inhibitory effects of resveratrol on NCI - H446 cells immigra- tion and invasion are, in part, associated with the NF- kB- mediated MMP -- 9 expression.

关 键 词：白藜芦醇 肺癌细胞 NCI-H446 基质金属蛋白酶 NF-ΚB 

分 类 号：R734.2[医药卫生—肿瘤]