三吲哚乙酸对Chlorella vulgaris XJB生长及产油能力的影响  

Effects of Indole-3-acetic Acid on the Growth and Lipid-Producing Capacity of Chlorella vulgaris XJB

作　　者：王思雨[1] 徐小琳[1] 贺莹莹[1] 代斌[1] 李春[1,2] 王长海[3] 

机构地区：[1]石河子大学化学化工学院/新疆兵团化工绿色过程重点实验室/省部共建国家重点实验室培育基地,石河子832003 [2]北京理工大学生命学院,北京100081 [3]南京农业大学资源与环境科学学院,南京210095

出　　处：《石河子大学学报（自然科学版）》2013年第4期499-503,共5页Journal of Shihezi University(Natural Science)

基　　金：国家科技支撑计划项目(2011BAD14B01)

摘　　要：三吲哚乙酸(IAA)具有促进细胞增殖的作用,将其添加到培养基中能够提升藻类细胞生长,可增强利用微藻发展生物柴油的能力。本文以自行筛选到的一株具有快速生长能力的微藻ChlorellavulgarisXJB为研究对象,考察了IAA添加时间和添加浓度对C.vulgaris XJB生长及产油能力的影响。实验研究结果表明,在微藻生长适应期,添加适宜浓度的IAA有助于藻体生物量的累积和油脂产率的提高,但对油脂含量的影响较小,当第0天添加0.08mg/L IAA时,细胞个数、生物量及油脂产率达到最大值,分别为20.2×106个/mL、338.9mg/L、15.2mg/(L/d),油脂含量为22.4%,此时生物量和油脂产率分别是未经IAA处理条件下的2.5倍和2.0倍。Indole-3-acetic acid（IAA）could promote cell proliferation and facilitate the growth of microalgae when it is added into medium. Thus,it could enhance the lipid productivity of microalgae in the development of biodiesel. In this paper, Chlorella vulgaris XJB with the characterization of fast growth was studied. Effects of IAA on the growth and lipid-producing capacity of C. vulgaris XJB were investigated under different adding time and concentrations. Results showed that IAA with the suitable concentration was contributed to enhance biomass and lipid productivity when it was added in adaptation period,but not helpful for lipid content. The highest cell number, hiomass and lipid productivity of C. vulgaris XJB reached 20.2 ×106 cell · mL-1 , 338.9 mg· L -1 ,15.2 mg · L-1 · d-1 ,respectively,with the lipid content of 22.4% when 0.08 mg · L-1 of IAA was added into medium on 0th day. The biomass and lipid productivity was 2.5 and 2 folds than that of control without IAA.

关 键 词：三吲哚乙酸 CHLORELLA VULGARIS 生物量 油脂含量 油脂产率 

分 类 号：TE667[石油与天然气工程—油气加工工程] Q945.3[生物学—植物学]