血液中游离DNA浓度和非小细胞肺癌相关性多因素分析  

Multivariate analysis of the relationship between free DNA concentration in the blood and prognosis of non-small cell lung cancer

作　　者：龚海峰[1] 刘俊峰[1] 

机构地区：[1]河北医科大学第四医院胸外科,石家庄050011

出　　处：《中国综合临床》2013年第10期1032-1035,共4页Clinical Medicine of China

摘　　要：目的探讨血液中游离DNA浓度和非小细胞肺癌预后等多因素的相关性。方法选取46例非小细胞肺癌患者（实验组）与21例非肺癌患者作为对照组，初诊时抽取患者10ml外周血，利用实时荧光定量PCR的方法针对人β-球蛋白基因来计算患者血浆中DNA的浓度，并进行为期6年的生存期跟踪调查；采用Kaplan—Meier方法和Cox回归对患者的生存期进行统计分析。结果实验组人群的中位DNA浓度显著高于对照组人群（52μg/L与29μg/L，P=0．03）。实验组死亡患者（87％）的中位DNA浓度明显高于仍然存活患者的中位DNA浓度（59mg／L与25mg／L，P=0．02）；病理分型、临床分期、患者年龄、性别、吸烟史和肺部炎症情况与血液中的DNA浓度无相关性（P均〉0．05）。结论非小细胞性肺癌患者在初始治疗前血液中DNA的浓度与患者预后有关，与非小细胞肺癌病理分型、临床分期、吸烟史和肺部炎症情况等多因素无关。Objective To investigate the association of circulating free blood DNA and prognosis of non-small cell lung cancer （NSCLC）. Methods Forty-six untreated NSCLC patients and 21 controls were selected as our subjects, and all underwent a 6 years follow-up. The level of circulating free blood DNA was determined by real-time quantitative polymerase chain reaction （qPCR） targeting the human β-globin gene. Overall survival of the patients were analyzed by Kaplan-Meier method and Cox-regression. Results At the end of follow-up,the median free DNA concentration of experimental patients was higher than that of control patients（52 μg/L vs. 29 μg/L, P = 0. 03 ）. The median free DNA concentration of experimental death patients （ 87% ） died was significantly higher than that of still survived patients （ 59 mg/L vs. 25 rag/L, P = 0. 02 ）. No association of DNA concentration with tumor pathology, clinical stage, age, gender, smoking or pulmonary inflammatory conditions were found in current study. Conclusion For NSCLC patients, the circulating free blood DNA level was negative correlation with the survival of the patients, suggesting that it may be utilized as a prognostic factor for survival analysis. But there is no relationship of free blood DNA concentration with tumor pathology, age, gender, smoking or puhnonary inflammatory conditions.

关 键 词：非小细胞肺癌 预后 血液 DNA 

分 类 号：R734.2[医药卫生—肿瘤]