食管癌P53与Ki67联合检测的临床意义  

Clinical significance of combined detection of P53 and Ki67 in patients with esophageal carcinoma

作　　者：侯向生[1] 王玉香[2] 郑慧禹[3] 赵冬峰[3] 

机构地区：[1]郑州大学第二附属医院胸外科,450014 [2]郑州大学第二附属医院病理科,450014 [3]郑州人民医院胸外科

出　　处：《中国综合临床》2013年第10期1091-1094,共4页Clinical Medicine of China

摘　　要：目的探讨P53、Ki67的表达在食管癌细胞增殖、侵袭和转移中的临床意义及相互关系。方法随机收取2006年1月至2008年5月食管癌切除标本并脉管瘤栓86例，作为瘤栓组。并随机抽取食管癌无瘤栓86例作为对照组。共计172例进行研究。应用免疫组化二步法检测172例食管癌患者P53、Ki67的表达。结果P53表达率在瘤栓组和对照组分别为66．3％（57／86）和51．1％（44／86），差异有统计学意义（x2=4．045，P〈0．05），≥＋＋阳性表达差异有统计学意义（X2=8．592，P〈0．05）；Ki67表达率在瘤栓组和对照组分别为76．7％（66／86）和60．5％（82／86），差异有统计学意义（X2=5，291，P〈0．05），≥＋＋阳性表达差异有统计学意义（x2=13．661，P〈0．01）；P53与Ki67在两组标本中的表达呈正相关（r=0．400，P=0．00）。瘤栓组P53、Ki67表达与癌细胞浸润深度（x2值分别为4．923、5．977，P均〈0．05）、淋巴结转移（x2值分别为3．945、5．794，P均〈0．05）、癌细胞分化程度（x2值分别为5．570、4．354，P均〈0．05）有关。结论P53、Ki67的过度表达在食管癌细胞的增殖、侵袭和转移中起一定作用，共同影响着食管癌的发生、发展过程，两者联合检测有助于食管癌的早期诊断，并可判断其恶性程度、侵袭、转移及预后。Objective To investigate the clinical significance of expression of P53, Ki67 in proliferation, invasion and metastasis of cancer cells of patients with esophageal carcinoma. Methods Eighty-six patients with esophageal carcinoma were selected as tumor group from January 2006 to May 2008. And another 86 patients without tumor were as control group. ABC-immunohistochemistry was used to detect the expression of P53 and Ki67 in 172 cases of patients with esophageal carcinoma. Results The expression of P53 in patients with esophageal carcinoma was different from that in control subjects （ X2 = 4. 045, P 〈 0.05 ）, and the P53 expression rate in two group were 66. 3 % （57/86） and 51.1% （44/86） respectively. The rate of P53 expression reaching to ＋ ＋ in two groups was also significant （ X2 = 8. 592, P 〈 0.01 ）. Ki67 expression in patients with esophageal carcinoma was higher than that of control patients （ 76. 7% （ 66/86 ） vs. 60. 5% （ 82/86 ）, X2 = 5. 291 ,P 〈 0. 05 ）. Additionally, there was significant difference regarding of Ki6 expression reaching to ＋ ＋ positive（ X2 = 13. 661, P 〈 0. 01 ）. Moreover, the expression of P53 and Ki67 were found to be a positively correlation（ r = 0. 400, P = O. 00 ）. The expression of P53 and Ki67 were related with invasion and differentiation of cancer cells （ x2 = 3. 945,5. 794 respectively, all P 〈 0. 05 ） and lymph node metastasis （ X2 = 5. 570,4. 354 respectively,all P 〈 0. 05 ）. Conclusion Over-expression of P53 and Ki67 were involved in the process of proliferation ,invasion and metastasis of cancer cells in esophageal carcinoma, which coherently impacted the occurrence and development of esophageal cancer. The combined detection of P53 and Ki67 seems to help the early diagnosis of esophageal cancer and evaluation of the degree of malignancy, invasion, metastasis and prognosis. Over-expression of P53, Ki67 might be served as a reference marker in screening for chemotherapy for esophageal cancer.

关 键 词：食管癌 P53 KI67 检测 

分 类 号：R735.1[医药卫生—肿瘤]