地榆鞣质对骨髓抑制小鼠MGMT基因及蛋白表达的影响  

Efect of Sanguisorba tannins on the expression of MGMT gene and protein in myelosuppressed mice

作　　者：熊永爱[1] 邹俊波[1] 王芳[2] 张帅杰[2] 杨明[1,2] 

机构地区：[1]成都中医药大学药学院中药炮制制剂研究所,四川成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《肿瘤》2013年第9期781-785,共5页Tumor

基　　金：国家自然科学基金资助项目(编号:81373976)

摘　　要：目的:观察地榆鞣质对骨髓抑制小鼠的DNA修复蛋白O6-甲基鸟嘌呤DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MGMT)基因及蛋白表达的影响。方法:40只昆明种小鼠按体质量随机分为正常对照组、模型组、阳性对照组和地榆鞣质组,每组10只。通过腹腔注射环磷酰胺(cyclophosphamide,CTX)诱导建立小鼠骨髓抑制模型。阳性组在CTX诱导3 d后按30μg/kg剂量经腹腔注射集落刺激因子,地榆鞣质组在CTX诱导前3 d开始按20 mg/kg剂量灌胃给予地榆鞣质水溶液,正常对照组和模型组均灌胃等体积纯净水。用药治疗结束后,收集血液和骨髓,FCM法测定骨髓细胞DNA含量,并采用反转录PCR(reverse transcriptionPCR,RT-PCR)和蛋白质印迹法测定骨髓细胞中MGMT基因及蛋白表达的变化。结果:与正常对照组比较,模型组小鼠骨髓DNA含量显著减少(P<0.01),骨髓细胞中MGMT基因及其蛋白表达也均显著减少(P<0.01)。与模型组比较,地榆鞣质可明显提高小鼠骨髓DNA含量(P<0.01),并明显促进骨髓细胞中MGMT基因及蛋白的表达(P<0.01)。结论:地榆鞣质可促进骨髓抑制小鼠骨髓细胞中MGMT基因及蛋白的表达。Objective： To observe the effect of Sanguisorba tannins on the expression of MGMT O^6-methylguanine-DNA methyltransferase） gene and protein in myelosuppressed mouse model induced by CTX （cyclophosphamide）. Methods： Forty Chinese Kunming mice were randomly divided into 4 groups： normal group （NR group）, model group （MD group）, positive group （G-CSF group） and Sanguisorba tannins group （ST group）, with 10 mice in each group. Mouse model of bone marrow suppression was made by intraperitoneal injection of CTX. The mice in G-CSF group were intraperitoneally injected with 30 μg/kg colony-stimulating factor after CTX induction for 3 days. The mice in ST group started to take 20 mg/kgSanguisorba tannins orally 3 days before CTX induction. The mice in NR group and MD group were given purified water orally. At the end of the treatment, blood and bone marrow of all mice were taken out. Then the flow cytometry technology was used to examine the DNA content in bone marrow cells. The RT-PCR （reverse transcription-PCR） and Western blotting technologies were used to determine the expression changes of MGMT gene and protein in bone marrow cells. Results： Compared with the NR group, the DNA content （P 〈 0.01） and the expressions of MGMT gene and protein （P 〈 0.01） were significantly reduced in bone marrow cells of mice in MD group. Compared with the MD group, bone marrow DNA content and the expression levels of MGMT gene and protein were significantly increased in ST group （P 〈 0.01）. Conclusion： Sanguisorba tannins can promote the expression of MGMT gene and protein in bone marrow cells of myelosuppressed mice.

关 键 词：抗肿瘤药(中药) 地榆 DNA损伤 血细胞生成 骨髓 O6-甲基鸟嘌呤DNA甲基转移酶 小鼠 

分 类 号：R730.52[医药卫生—肿瘤]