腹直肌-腹膜瓣修复舌体缺损动物模型的建立  

Establishment of animal model of rectus abdominis musculoperitoneal flap to reconstruct tongue defect

作　　者：李军[1] 钟良军[1] 木合塔尔.霍加 多力昆[2] 闫广鹏[2] 

机构地区：[1]新疆医科大学第一附属医院颌面外科,乌鲁木齐830054 [2]新疆维吾尔自治区人民医院颌面外科,乌鲁木齐830001

出　　处：《华西口腔医学杂志》2013年第5期448-452,共5页West China Journal of Stomatology

基　　金：新疆维吾尔自治区自然科学基金资助项目(2010211A54)

摘　　要：目的探讨携带神经的腹直肌-腹膜瓣移植修复舌体缺损的可行性。方法研究选用12只Beagle犬,随机分为3组,制备带神经的腹直肌-腹膜瓣修复舌体缺损的动物模型,其中2组(A、B组)行肋间神经与舌下神经吻合,另一组不行神经吻合。左侧为实验侧,右侧为正常对照侧。术后12周应用激发肌电图,检测舌下神经潜伏期、振幅及传导速度,观察腹直肌-腹膜瓣是否重获舌下神经再支配。结果 12只Beagle犬中9只带神经的腹直肌-腹膜瓣修复舌体缺损的动物模型建立成功,1只雄性犬术后第3天因腹疝死亡,2只雌性犬因动脉解剖与雄性犬差异较大,腹壁下动脉与舌动脉管径相差较大,无法吻合。AB组舌下神经传导速度分别恢复至正常侧的40%、30%。结论采用带神经的腹直肌-腹膜瓣移植修复舌体缺损具有可行性,失神经腹直肌-腹膜瓣可重获舌下神经的支配,舌下神经支配功能可部分恢复。Objective This study aims to investigate the feasibility of tongue reconstruction by a rectus abdominis muscu- lopefitoneal flap with neurovascular pedicled in a canine model. Methods Twelve Beagle dogs were enrolled to the experi- ment. The animals were randomly divided into three groups, two of which （group A and B） had nerve anastomosis. The left sides were experimental sides, whereas the fight sides were control sides. Twelve weeks after operation, electrophysiological test was performed to detect hypoglossal nerve latency amplitude and conduction velocity as well as to evaluate the reinner- vation of the rectus abdominis musculoperitoneal flap. Results Among the 12 Beagle dogs, nine animal tongue reconstruc- tion models by rectus abdominis musculoperitoneal flap with neurovascular pedicled were successful, whereas one male Beagle dog died from ventral hernia 3 d after the operation, two female rectus peritoneal flaps were abandoned because their arterial anatomy differed from the male, which was not ideal. Hypoglossal nerve conduction velocity of group A and B were restored to the normal side of the 40%, 30%. Conclusion Animal models of tongue reconstruction can be established by a rectus abdo- minis musculoperitoneal flap with neuruvascular pedicled in Beagle dogs. Denervated rectus abdominis musculopefitoneal flap can regain hypoglossal nerve innervation. Hypoglossal nerve functions partly recover.

关 键 词：腹直肌-腹膜瓣 舌下神经 动物模型 肌电图 

分 类 号：R-332[医药卫生]