雌激素受体与Ⅱ型胶原在人胚髁突软骨中的表达  

Expression of the estrogen receptor and type Ⅱ collagen in the mandibular condylar cartilage of the human embryo

作　　者：张跃蓉[1] 庞磊[1,2] 刘忠林[1] 

机构地区：[1]遵义医学院口腔学院口腔解剖生理教研室,遵义563003 [2]湖北医药学院人体解剖教研室,十堰442000

出　　处：《华西口腔医学杂志》2013年第5期526-529,共4页West China Journal of Stomatology

基　　金：贵州省科学技术基金资助项目[黔科合J字(2010)2170号]

摘　　要：目的探讨雌激素受体(ER)和Ⅱ型胶原在人胚髁突软骨中的表达情况。方法采用苏木精-伊红(HE)及免疫组织化学染色法检测ERα、ERβ和Ⅱ型胶原在人胚颞下颌关节髁突软骨各层的表达。结果Ⅱ型胶原主要表达于髁突软骨的过渡层及肥大层。ERα主要表达于髁突软骨的过渡层及肥大层,均匀分布于细胞内;ERβ集中表达于肥大层,且以细胞核内分布为主。纤维层及增殖层未见Ⅱ型胶原和ER表达,钙化软骨层有少量表达。结论 ER与Ⅱ型胶原在髁突软骨中分布一致,雌激素可选择性地结合不同亚型的ER,从而调节髁突软骨细胞分泌Ⅱ型胶原的能力。Objective To study the expression of the estrogen receptor （ER） and type II collagen in the mandibular con- dylar cartilage of the human embryo. Methods The expression and localization of ERos, ERr, and type II collagen in the mandibular condylar cartilage of the human embryo were examined through hematoxylin-eosin （HE） and immunohistoche- mistry staining. Results Type lI collagen was primarily localized in the transitional and hypertrophic layers of the condylar cartilage. ERa was mostly expressed in the transitional and hypertrophic cartilaginous layers of the condylar cartilage. ERct was evenly distributed in the cell, whereas ERr was localized in the nuclei. No expression of type II collagen and ER was found in the fibrage and the proliferative layer although minimal expression was found in the calcified cartilage. Conclusion The distribution of ER and type II collagen in the mandibular condylar cartilage was consistent. Estrogen can selectively combine with different subtypes of ER that regulate the ability of the condylar cartilage cells to secrete type II collagens.

关 键 词：髁突软骨 雌激素 雌激素受体 人胚 

分 类 号：R780.2[医药卫生—口腔医学]