喉鳞状细胞癌组织中Caspase-3的表达及其与bFGF和微血管密度的关系  

The expression of Caspase-3,bFGF and MVD in laryngeal squamous cell carcinoma and the relationship among them

作　　者：徐勤[1] 高珊[2] 刘吉娜[2] 

机构地区：[1]承德医学院附属医院肿瘤放化疗科,河北承德067000 [2]承德医学院附属医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2013年第19期1084-1087,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨喉癌中Caspase-3、bFGF的表达以及在新生血管生成过程中的作用。方法:应用免疫组织化学SP法分别检测67例喉鳞状细胞癌组织和36例癌旁组织中Caspase-3和bFGF两种蛋白的表达情况,并用CD34标记并计数微血管密度(MVD)。结果:bFGF在喉鳞状细胞癌组织中的表达高于癌旁组织,Caspase-3的表达低于癌旁组织(P<0.05);喉鳞状细胞癌组织中bFGF和Caspase-3的表达在不同分化程度之间、淋巴结有无转移及不同临床分期的差异有统计学意义(P<0.05),在不同临床分型、有无吸烟史、不同性别及年龄方面的差异无统计学意义(P>0.05)。bFGF与Caspase-3在喉癌组织中表达呈显著负相关(r=-0.523,P<0.05)。喉癌中MVD值明显大于喉癌旁黏膜(P<0.05),并与淋巴结转移显著相关(P<0.05),与不同分化程度、不同临床分期、不同临床分型、有无吸烟史、不同性别及年龄方面的差异无统计学意义(P>0.05)。喉鳞状细胞癌组织中bFGF蛋白表达阳性组MVD值,显著高于bFGF阴性表达组MVD值(P<0.05)。Caspase-3蛋白表达阴性组MVD与Caspase-3蛋白表达阳性组MVD值比较,二者差异无统计学意义(P>0.05)。结论:bFGF和Caspase-3关系密切,二者在喉鳞状细胞癌的表达与喉癌的临床病理学特征密切相关,二者的检测将有助于喉癌的诊断、评估喉癌患者的恶性程度及预后。Objective：To investigate the expression of Caspase-3 and bFGF in laryngeal squamous cell carcino- ma and the effecton angiogenesis. Method： Immunohistochemistry was used to detect the expression of Caspase-3, bFGF and MVD in 36 eases of para-carcinoma tissue and 67 cases of laryngeal squamous cell carcinoma. Result： The expression of bFGF in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma tis- sue（P〈0.05）, however the expression of Caspase-3 was significantly higher in para-carcinoma tissue than that in laryngeal squamous cell carcinoma（P〈0.05）. In laryngeal carcinoma, the expression of BFGF and Caspase-3 were associated with differentiation, lymph node metastasis and clinical stage（P〈0. 05）, but was independent of clini- cal classification,smoking history, sex and gender （P〈0.05）. A signifcantly negative correlation was found be- tween bFGF and Caspase-3（P〈0.05）. The value of MVD in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma tissue（P〈0.05）, and was significantly associated with lymph node metastasis （P〈0.05）, but not associated with age, gender, differentiation, clinical stage, clinical classification or smoking history. The MVD of the tissue with positive expression of bFGF was significantly higher than that with negative expression of bFGF in laryngeal squamous cell carcinoma（P〈0.05）. There was no significant difference between the MVD of the tissue with positive expression of bFGF and that with negative expression of bFGF in laryngeal squamous cell earcinoma（P〈0. 05）. Conclusion： bFGF was positively related to Caspase-3, which might play an important role in the carcinogenesis and development of laryngeal carcinoma by synergic effect.

关 键 词：BFGF Caspase-3 微血管密度 喉肿瘤 癌 鳞状细胞 

分 类 号：R739.65[医药卫生—肿瘤]