针对fimW基因沙门氏菌的特异性PCR检测  

Simple and rapid detection of Salmonella by direct PCR amplification of the fimW gene

作　　者：张江英[1] 董立伟[1] 王小舟[1] 朱国强[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国预防兽医学报》2013年第10期837-839,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31101833;31101826;31270171);扬州市农业科技攻关计划项目(yz2011066)

摘　　要：为建立沙门氏菌快速检测方法，本研究根据沙门氏菌I型菌毛蛋白调控基因胁w设计一对引物，建立PCR检测方法，并对收集的A群～F群14个血清型40株沙门氏菌和5种12株非沙门氏菌菌株进行PCR扩增。结果显示所有沙门氏菌均扩增出与预期（477bp）相符的特异性片段，而非沙门氏菌扩增结果均为阴性。另外，以肠炎沙门氏菌50336株DNA为模板，敏感性试验结果显示该方法可以检测出扩增体系中lpgDNA染色体和10-2 cfu的沙门氏菌。表明本研究建立了检测沙门氏菌简洁、敏感、特异的PCR方法。In the present study, a specific PCR method for detecting Salmonella species was established with a pair of primers designed to according to the timW gene, which encoded a protein involved in regulating Salmonella type I fimbriae expression. The detection results showed that the 477 bp specific DNA fragments were amplified from all of 40 Salmonella strains of 14 serovars with a detection limit of lpg genomic DNA or 102 cfu in the amplifying system with the template DNA from S.enteritis 50336 strain, but no any amplification from 12 non-Salmonella bacteria strains of 5 species. Therefore, the established method is a simple, sensitive and specific for Salmonella detection.

关 键 词：沙门氏菌 检测 fimW基因 PCR 

分 类 号：S852.61[农业科学—基础兽医学]