Twist蛋白在肾肿瘤组织中的表达及与MRP、P-gp、LRP、TOPOⅡ的关系  

The expression of Twist protein in renal carcinoma and its relationship to MRP, P- gp, LRP, and TOPO Ⅱ expressions

作　　者：张志霞[1] 何涛[2] 董衍东[2] 王丽[2] 

机构地区：[1]枣庄职业学院医学系,山东枣庄277800 [2]泸州医学院分子生物学实验室,四川泸州646000

出　　处：《徐州医学院学报》2013年第9期588-591,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的 探讨Twist蛋白在肾肿瘤组织中的表达及与几种多药耐药蛋白表达之间的关系.方法 应用免疫组化SABC技术检测人肾癌组织不同区域的Twist蛋白、多药耐药相关蛋白（multidrug resistance-associated protein,MRP）、P糖蛋白（P-gp）、肺耐药蛋白（lung resistance-related protein,LRP）、拓扑异构酶Ⅱ（topoisomerase Ⅱ,TOPOⅡ）的表达.结果 在肿瘤边界区,Twist蛋白、MRP、P-gp、LRP、TOPOⅡ均为阳性表达;而在肿瘤中心区,Twist蛋白和P-gp的表达为阴性,MRP、LRP、TOPOⅡ蛋白仍为阳性表达,但MRP和TOPOⅡ在肿瘤中心区表达强于肿瘤周边区（P〈0.01）,而LRP在肿瘤中心区的表达弱于肿瘤周边区（P〈0.01）.结论 Twist基因的表达与肾肿瘤转移、浸润以及耐药性有关.Objective To investigate the relationship between the expressions of Twist protein in renal carcinoma tissue and several drug resistance proteins. Methods The expressions of Twist protein, multidrug resistance - associated protein （MRP）, P- glycoprotein （P- gp）, lung resistance protein （LRP）, and topoisomerase Ⅱ （TOPO Ⅱ） in different zones of human renal carcinoma tissues were detected using streptavidin - biotin - peroxidase complex （SABC） immune histochemistry technique. Results Immunostaining results showed that the expressions of Twist protein, MRP, P - gp, LRP, and TOPO Ⅱ were positive in the boundary zone of renal carcinoma, while in the cancer center zone, the expressions of Twist protein and P - gp were negative. MRP, LRP, and TOPO Ⅱ were still positively expressed in cancer center zone, and the expression levels of MRP and TOPO Ⅱ were higher in cancer center zone than those in boundary zone （P 〈0.01 ）, while the expression level of LRP was lower than that in boundary- zone （P 〈0. 01 ）. Conclusion Twist gene is related to metastasis, infiltration, and drug resistance in renal carcinoma.

关 键 词：肾肿瘤 Twist 多药耐药相关蛋白 P糖蛋白 肺耐药蛋白 拓扑异构酶Ⅱ 

分 类 号：R735.7[医药卫生—肿瘤]