水稻Ef7基因的一个新等位基因Ef7-l的遗传效应及表达分析  

Genetic and Expression Analyses of Ef7-l,a Novel Ef7 Allele,in Rice

作　　者：赵冬生[1] 张昌泉[1] 顾铭洪[1] 刘巧泉[1] 

机构地区：[1]扬州大学农学院/江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009

出　　处：《作物学报》2013年第10期1799-1805,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2012AA10A302-7);国家自然科学基金项目(31071383);江苏省杰出青年基金项目(BK2012010);江苏省作物学优势学科项目资助

摘　　要：水稻Ef7基因控制抽穗期。本研究从我国籼稻品种龙特甫中克隆了Ef7的等位基因Ef7-l,序列比对表明,Ef7-l编码序列与日本晴Ef7相比存在5个氨基酸的差异。利用两个等位基因的序列差异,以日本晴为受体构建了含有Ef7-l的近等基因系CL63。CL63在长日照条件下比轮回亲本日本晴延迟抽穗约6 d,但在短日照条件下两者抽穗期无显著差异。田间性状分析显示Ef7-l等位基因在长日照条件下能够使植株茎秆变粗,粒重显著增加,这可能是通过上调OsPHYB的表达水平而延迟水稻抽穗期实现的。Ef7 controls rice heading date.In the present study,an allele of Ef7,Ef7-l,was cloned from the indica rice cultivar Longtefu.Sequence analysis indicated that Ef7-l is a novel Ef7 allele and there are five amino acid substitutions in Ef7-l compared with its wild type from Nipponbare.Based on the sequence variation between Ef7-l and Ef7,we developed a near-isogenic line（NIL）CL63 with the background of japonica Nipponbare via marker-assisted selection.The heading date of the NIL CL63 was postponed for approximate six days under long day（LD）condition compared with Nipponbare.In short day（SD）condition,there was no obvious difference in heading date between Nipponbare and CL63.Under the natural field at Yangzhou,the novel allele Ef7-l could result in significant increase of plant height and grain weight in CL63 compared with Nipponbare.The qRT-PCR analysis revealed that there was not significant difference in Ef7 gene expression level between Nipponbare and CL63 under both LD and SD conditions.But the Ef7-l allele could affect rice heading date by up-regulating the expression level of OsPHYB gene under LD condition.

关 键 词：水稻 抽穗期 Ef7-l 等位基因 表达分析 

分 类 号：S511[农业科学—作物学]