Protein Expression in Silica Dust-induced Transdifferentiated Rats Lung Fibroblasts  

作　　者：HAO Chang Fu LI Xiao Fang YAO Wu 

机构地区：[1]Department of Occupational Health and Environmental Health,College of Public Health,Zhengzhou University

出　　处：《Biomedical and Environmental Sciences》2013年第9期750-758,共9页生物医学与环境科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(#81102109);the Basic Research and Frontier Technology Projects of Henan Province(#72300450190)

摘　　要：Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibrob^asts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in a-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis （2-DE）. Results Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin. Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblastsObjective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibrob^asts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in a-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis （2-DE）. Results Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin. Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts

关 键 词：Transdifferentiation  Myofibrob^ast Two-dimensional electrophoresis Crystalline silica 

分 类 号：R363[医药卫生—病理学]